PreadyPort


 

A NOVEL READY-TO-USE SYSTEM FOR IN VITRO DRUG TRANSPORTER EVALUATION

Membrane transporters can be major determinants of the pharmacokinetic, safety and efficacy profiles of drugs. Monolayer assays using transfected MDCKII-MDR1/P-gp or BCRP (ABCG2) cells have been widely recognized tools to study the interaction of drugs with transporters. SOLVO Biotecnology and Readycell introduce PreadyPort™, the Ready-to-use Transfected MDCKII Monolayer Kits containing differentiated MDCKII cells expressing the selected drug transporter on 24- or 96-well cell culture insert plates.

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PreadyPort-MDR1 Go to PreadyPort-BCRP











A NOVEL READY-TO-USE SYSTEM FOR IN VITRO DRUG TRANSPORTER
SOLVO Biotechnology & READYCELL introduce PreadyPort™ in collaboration

  • PreadyPort™ is a line of products based on MDCKII cells expressing different drug transporters
  • Study substrate and inhibitor interactions of drugs with MDR1/Pgp transporter
  • Model the net transport events of barriers showing high MDR1/Pgp expression such as the blood-brain barrier and the intestine

PreadyPort™-MDR1 Kit contains 24 & 96 insert-integrated plates with differentiated MDCKII cells expressing MDR1, as well as the parental cell line. The first member of the PreadyPort series, the PreadyPort™-MDR1 Kit is revolutionary in providing a ready-to-use tool for MDCKII-MDR1 monolayer assays. The kit will allow researchers to perform MDR1 interaction studies on monolayers without worrying about cell-line licensing and culturing.

The patented shipping medium allows storage and transportation at room temperature, without altering cell functional properties.

 

PreadyPort line complies with regulatory recommendations from The International Transporter Consortium (Nature Reviews Drug Discovery, 9, 2010)

FEATURES & BENEFITS
  • Low variability/high reproducibility: the entire system is tested for batch-to-batch consistency and qualified for performing transport-related assays so that the system can be used with total confidence
  • Maximum convenience: High flexibility as the kit is useable up to 7 days upon receiving.
  • Available under a limited single-user license without extra charge
  • Time saving and cost-effective
  • Automation compatible: Adaptable to automated procedures and High Throughput Screening
  • User-friendly system and clean handling
  • Labor saving: Provides differentiated MDCKII-MDR1 barrier (11 day system) allowing reduction of in-house cell propagation and cell line development or acquisition
  • Shipping medium easy to remove by liquefaction at 37ºC

Stability of PreadyPort™-MDR1 barrier properties under shipping conditions

The barrier stability of the PreadyPort™-MDR1 kit during shipping and storage is presented above. The 11-day MDCKII-WT and MDR1 monolayers were maintained in shipping medium for 1, 2, 3 and 4 days, then their barrier status was evaluated by TEER (trans-epithelial electrical resistance) measurement before applying and 24 hours after removing the shipping medium. Cell monolayers showed no changes in barrier properties up to 4 days in the presence of shipping medium indicating that PreadyPort™-MDR1 can be stored and transported at room temperature up to 4 days without loss of its barriers functions.

  • Functional Stability of PreadyPort™-MDR1 in 24 & 96-insert well plate formats

    Pgp/MDR1-mediated digoxin transport was determined from three independent experiments at day 12 of culture in both, 24 and 96-insert integrated plates formats. Transport of digoxin was specifically inhibited by PSC833 Pgp inhibitor.

  • Preadyport™-MDR1-96: Concentration dependence of Digoxin Transport Inhibition by PSC833

Complete inhibition curve of MDR1-mediated digoxin transport at different concentrations of PSC833 (specific MDR1 inhibitor).

  • PreadyPort™ product line will be extended with MDCKII-BCRP and MDCKII-OATP2B1/BCRP kits with their respective 96-well plate versions in the near future
QUALITY CONTROL

Efflux Ratio Values for Known Pgp/MDR1 Substrates and Inhibitors in 24 & 96-insert well formats

Efflux ratio values of transport of different MDR1/Pgp substrates obtained with PreadyPortTM-MDR1 in 24 and 96-well formats
FORMATS
  • PreadyPort™-MDR1: MDCKII-MDR1 cells on 24-insert well plate - Ref. KRECE-MDR01.
  • PreadyPort™-CTRL: MDCKII parental cells (CTRL) on 24-insert well plate - Ref. KRECE-CTR01.
  • PreadyPort™-MDR1/CTRL: 50% MDR1 - 50% CTRL cells on 24-insert well plate - Ref. KRECE-MDR02.
  • PreadyPort™-MDR1: MDCKII-MDR1 cells on 96-insert well plate - Ref. KRECE-MDR050.
  • PreadyPort™-CTRL: MDCKII parental cells (CTRL) on 96-insert well plate- Ref. KRECE-CTR50.
  • PreadyPort™-MDR1/CTRL: 50% MDR1 - 50% CTRL cells on 96-insert well plate - Ref. KRECE-MDR51.
  • PreadyPort™-MDR1/CTRL: 75% MDR1 - 25% CTRL (3:1) cells on 96-insert well plate - Ref. KRECE-MDR52.

    NEW! Customized Design for 96 insert-integrated plate:

  • Custom setup: PreadyPort™-MDR1/CTRL Custom setup on 96 insert well plate - Ref. KRECE-MDR60

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A NOVEL READY-TO-USE SYSTEM FOR IN VITRO DRUG TRANSPORTER EVALUATION
SOLVO Biotechnology & READYCELL introduce PreadyPort™-BCRP in collaboration

  • PreadyPort™ is a line of products based on MDCKII cells expressing different drug transporters
  • Study substrate and inhibitor interactions of drugs with BCRP transporter
  • Model the net transport events of barriers showing high BCRP expression such as the intestine, hepatic and blood-brain barriers.

PreadyPort™-BCRP Kit contains 24 insert-integrated plates with differentiated MDCKII cells expressing BCRP, as well as the parental cell line. PreadyPort Kits are revolutionary in providing a ready-to-use tool for MDCKII-BCRP monolayer assays. The kit will allow researchers to perform BCRP interaction studies on monolayers without worrying about cell-line licensing and culturing.

The patented shipping medium allows storage and transportation at room temperature, without altering cell functional properties.
FEATURES & BENEFITS
  • Low variability/high reproducibility: the entire system is tested for batch-to-batch consistency and qualified for performing transport-related assays so that the system can be used with total confidence
  • Maximum convenience: High flexibility as the kit is useable up to 7 days upon receiving.
  • Available under a limited single-user license without extra charge
  • Time saving and cost-effective
  • Automation compatible: Adaptable to automated procedures and High Throughput Screening
  • User-friendly system and clean handling
  • Labor saving: Provides differentiated MDCKII-BCRP barrier (11 day system) allowing reduction of in-house cell propagation and cell line development or acquisition
  • Shipping medium easy to remove by liquefaction at 37°C

Stability of PreadyPort™-MDR1 barrier properties under shipping conditions

The barrier stability of the PreadyPort™-BCRP kit during shipping and storage is presented above. The 11-day MDCKII-WT and BCRP monolayers were maintained in shipping medium for 1, 2, 3 and 4 days, then their barrier status was evaluated by TEER (trans-epithelial electrical resistance) measurement before applying and 24 hours after removing the shipping medium. Cell monolayers showed no changes in barrier properties up to 4 days in the presence of shipping medium indicating that PreadyPort™-BCRP can be stored and transported at room temperature up to 4 days without loss of its barriers functions.

High Flexibility of PreadyPort™-BCRP

    The shipping medium was maintained for 4 days at room temperature. After removed, TEER was measured after 3 (D11) and 7 (D15) days of standard culture conditions. Results represent the average of three independent experiments.

  • Functional Stability of PreadyPort™-BCRP in 24-insert well plate format

    BCRP-mediated prazosin transport was determined from three independent experiments at day 12 of culture in 24-insert integrated plate format. Transport of prazosin was specifically inhibited by Ko134 BCRP inhibitor. No transport was seen in MDCKII-wt cells.

  • PreadyPort™ product line will be extended with MDCKII-OATP2B1/BCRP kits with their respective 96-well plate versions in the near futur

QUALITY CONTROL
  • Papp values obtained with PreadyPortTM-BCRP after the incubation of different BCRP substrates and the specific BCRP inhibitor Ko143
Papp values obtained after the incubation of Prazosin alone or in the presence of a BCRP specific inhibitor Ko143. Papp values for prazosin were not altered by the presence of 50 µM of Cerivastatine

Papp values obtained after the incubation of Chlorothiazide alone or in the presence of a BCRP specific inhibitor Ko143 or Cerivastatine

Results of the transport of Dantrolene alone or in the presence of Ko143 after 1 and 2h of incubation on MDCKII-wt and MDCKII-BCRP monolayers.
  • Efflux Ratio Values for Known BCRP Substrates and Inhibitors in 24-insert integrated well plates

Efflux ratio values of different BCRP substrates obtained with PreadyPortTM-BCRP in 24 insert-integrated well plates. In the presence of the specific BCRPinhibitor Ko143the efflux ratio decreased to values around 1.
FORMATS
  • PreadyPortTM-BCPR: MDCKII-BCRP cells on 24-insert well plate - Ref. KRECE-BCP01.
  • PreadyPortTM-CTRL: MDCKII parental cells (CTRL) on 24-insert well plate - Ref. KRECE-CTR01.
  • PreadyPortTM-BCRP/CTRL: 50% BCRP - 50% CTRL cells on 24-insert well plate - Ref. KRECE-BCP02.

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