IsoCyp-Tox


 


A NOVEL CELL-BASED AND READY-TO-USE CONCEPT FOR IN VITRO EVALUATION OF BIOTRANSFORMATION-MEDIATED METABOLIC TOXICITY

    Do you know if your compound is biotransformed by a given CYP450?
    Do you know if the metabolite produced is toxic?
  • Early detection and screening of potential bioactivation-dependent acute toxicity of target compounds.
  • Identification of CYP isoforms involved in potential production of toxic metabolites.
  • Metabolic activation screening

IsoCyp™-Tox is an innovative cell-based and ready-to-use kit. The kit consists of 96-well plates seeded with the hepatic cell line HepG2 transiently transduced with individual human CYP450 isoforms, making these cells metabolically competent. IsoCyp™-Tox allows in vitro detection and screening of biotransformation-mediated toxicity of lead and drug targets in just one single experiment. IsoCyp™-Tox is a powerful as well as time and cost-saving tool during hit to lead and lead optimization phases in early stage of drug discovery and development.

The patented shipping medium allows storage and transportation at room temperature, maintaining cell functional properties.
FEATURES & BENEFITS
  • Labor saving: Provides a whole plate of HepG2 cells transduced with one single CYP or different columns transduced with different CYPs on demand (3A4, 1A2, 2E1, 2A6), allowing reduction of in-house maintenance and handling of cell cultures
  • Maximum convenience: Isocyp™-Tox provides a simple viability/ toxicity end point. Plate is ready to perform your assay
  • Time saving and cost-effective
  • Automation compatible: Adaptable to automated procedures and High Throughput Screening.
  • User-friendly system and clean handling
  • Shipping medium easy to remove by liquefaction at 37ºC
  • Low variability / High reproducibility: The entire system is tested for batch-to-batch consistency and qualified for toxicity evaluation assays so that the system can be used with total confidence


QUALITY CONTROL

Functionality of IsoCyp™-Tox evaluated by endpoint WST-1 assay, measuring CYP bioactivation through different reference compounds
FORMATS

IsoCyp™-Tox standard kit containing 5 x 96-well plates, Ref. KRECE-ICT50:

  • 4 x 96-well plates of HepG2 transduced with single CYP450 isoform, 1 x 96-well plate for each CYP450 isoform (3A4, 2E1, 1A2 and 2A6)
  • 1 x 96-well control plate of HepG2 untransduced (metabolic incompetent cells)
IsoCyp™-Tox individual CYP450 kit containing 2 x 96-well plates:
  • 1 x 96-well plate of HepG2 transduced with single CYP450 of interest: 3A4 - Ref. KRECE-ICT51; 2E1 - Ref. KRECE-ICT52; 1A2 - Ref. KRECE-ICT53 or 2A6 - Ref. KRECE-ICT54
  • 1 x 96-well control plate of HepG2 untransduced (metabolic incompetent cells)