CacoGoblet


 


A MUCUS SECRETING READY-TO-USE CO-CULTURE OF CACO-2 AND GOBLET CELLS FOR IN VITRO INTESTINAL ABSORPTION EVALUATION

  • Specific for compounds that cross the intestinal barrier in a paracellular passive diffusion way.
  • Specific for compounds that could interact with mucus.
  • Evaluation of: oral absorption efficiency, oral bioavailability and oral toxicity
  • Study of mechanisms involved in oral and intestinal absorption
  • Suitable for research on new delivery system

CacoGoblet consists of 24 and 96 insert supports, seeded with differentiated polarized Caco-2 and human goblet cells on polycarbonate microporous filters. CacoGoblet allows in vitro intestinal absorption evaluation of drug targets in a barrier physiologically closer to the intestinal epithelium than standard Caco-2 monolayer models. CacoGoblet is flexible, since plates can be used up to five days after ideal cell barrier differentiation at day 21.

The patented shipping medium allows storage and transportation at room temperature, without altering cell functional properties.
FEATURES & BENEFITS
  • CacoGoblet represent a more permissive barrier model that mimics the physiological conditions of the intestinal epithelium

  • Low variability / High reproducibility: The entire system is tested for batch-to-batch consistency and qualified for use with compounds or formulations with passive diffusion transport pathway so that the system can be used with total confidence

  • Maximum convenience: High flexibility as the kit is useable up to 5 days after 21-day cell barrier differentiation
  • User-friendly system and clean handling
  • Labor saving: Provides 21-day cell barrier allowing reduction of in-house maintenance and handling of Caco-2 and Goblet cell cultures
  • Shipping medium easy to remove by liquefaction at 37ºC
  • Time saving and cost-effective
  • Full cell functionality after transportation
QUALITY CONTROL

Functionality of CacoGoblet barrier in comparison with CacoReadyTM evaluated by permeability assays of several reference compounds at day 21 of culture.
FORMATS

CacoGoblet kit provides the standard 21-day Caco-2 and human goblet cell barrier on 24 and 96 insert-integrated plates. The kit is shipped at day 14 of differentiation and after shipping medium application. Finally, plates provide a 21-day barrier.

CacoGoblet cell based assay, 24 insert-integrated plate:

  • Costar plate, Ref. KRECE-CCG01

CacoGoblet cell based assay, 96 insert-integrated plate:

  • Millipore plate, Ref. KRECE-CCG50