Protein expression

Protein expression systems for expression in e. coli, yeast and mammalian cells

Expression in E. coli

Expression vectors for protein expression in E. coli

QuickLyse pEAS1-a expression vector

Price (€): 560

Applications

Expression of recombinant proteins in E. coli
One-step cell lysis and release of protein into supernatant
Compatible with all purification tags

Benefits

Recombinant proteins are purified directly from the culture supernatant
No need for lysozyme treatment or sonication
Gentle extraction conditions ensure integrity and activity of the recombinant protein
Suitable for high-throughput protein expression

Expression vectors for Escherichia coli

Applications

Tightly regulated expression of recombinant proteins in E. coli
Fermentation studies
Antibiotic-free cultivation using parDE operon

Benefits

Ultra-tight regulation and high levels of expression due to Pm/xylS regulon
Compatible with most expression vectors, including pET, pUC and pBAD series
Wide choice of antibiotic resistance markers
RK2 origin allows vector propagation in more than 60 gram negative species

Price (€):	Product name	Description
410	pVB1 expression vector	Promoter: Pm/xylS \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: -
410	pVB2 expression vector	Promoter: Pm/xylS \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: -
410	pVB3 expression vector	Promoter: Pm/xylS \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: -
410	pVB4 expression vector	Promoter: Pm/xylS \| Origin: RK2 \| Resistance 1: Tetracyclin \| Resistance 2: -
410	pVB5 expression vector	Promoter: Pm/xylS \| Origin: RK2 \| Resistance 1: Kanamycin \| Resistance 2: -
410	pVB6 expression vector	Promoter: - \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: Chloramphenicol
410	pVB7 expression vector	Promoter: - \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: Tetracyclin
410	pVB8 expression vector	Promoter: - \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: Kanamycin
410	pVB9 expression vector	Promoter: - \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: parDE

Expression in yeast

Expression system for protein expression in yeast

KickStart protein expression kit

Price (€): 970

Applications

Expression of recombinant proteins in S. cerevisiae
Expression of membrane proteins and secreted proteins

Benefits

Protein expression in rich medium (YPD)
Self-inducing system, no addition of inducer required
Protease deficient strain for higher protein yields
One-step purification

Yeast expression vectors

Applications

Expression of recombinant proteins in S. cerevisiae
Expression of membrane proteins and secreted proteins
Inducible expression

Price (€):	Product name	Description
560	p427-TEF yeast expression vector	TEF promoter \| High copy number plasmid \| G418 selection
560	p417-CYC yeast expression vector	CYC1 promoter \| Low copy number plasmid \| G418 selection
560	pTEF-MF yeast expression vector	TEF1 promoter \| High copy number plasmid \| Secretion of protein
560	pGAL-MF yeast expression vector	GAL1-L promoter \| High copy number plasmid \| Secretion of protein

pFunk yeast expression vectors
	General purpose yeast expression vectors Wide choice of promoters and auxotrophic markers Constitutive and inducible expression Rich multiple cloning site for easy subcloning of cDNA inserts

Price (€):	Product name	Description
560	p413CYC yeast expression vector	Promoter: CYC1 \| Origin: CEN/ARS \| Marker: HIS3
560	p413GAL-S yeast expression vector	Promoter: GAL1-S \| Origin: CEN/ARS \| Marker: HIS3
560	p413GAL-L yeast expression vector	Promoter: GAL1-L \| Origin: CEN/ARS \| Marker: HIS3
560	p414CYC yeast expression vector	Promoter: CYC1 \| Origin: CEN/ARS \| Marker: TRP1
560	p414TEF yeast expression vector	Promoter: TEF1 \| Origin: CEN/ARS \| Marker: TRP1
560	p414GAL-S yeast expression vector	Promoter: GAL1-S \| Origin: CEN/ARS \| Marker: TRP1
560	p414GAL-L yeast expression vector	Promoter: GAL1-L \| Origin: CEN/ARS \| Marker: TRP1
560	p415CYC yeast expression vector	Promoter: CYC1 \| Origin: CEN/ARS \| Marker: LEU2
560	p415ADH yeast expression vector	Promoter: ADH1 \| Origin: CEN/ARS \| Marker: LEU2
560	p415GAL-S yeast expression vector	Promoter: GAL1-S \| Origin: CEN/ARS \| Marker: LEU2
560	p415GAL-L yeast expression vector	Promoter: GAL1-L \| Origin: CEN/ARS \| Marker: LEU2
560	p416CYC yeast expression vector	Promoter: CYC1 \| Origin: CEN/ARS \| Marker: URA3
560	p416GAL-S yeast expression vector	Promoter: GAL1-S \| Origin: CEN/ARS \| Marker: URA3
560	p416GAL-L yeast expression vector	Promoter: GAL1-L \| Origin: CEN/ARS \| Marker: URA3
560	p423CYC yeast expression vector	Promoter: CYC1 \| Origin: 2micron \| Marker: HIS3
560	p423GAL-S yeast expression vector	Promoter: GAL1-S \| Origin: 2micron \| Marker: HIS3
560	p423GAL-L yeast expression vector	Promoter: GAL1-L \| Origin: 2micron \| Marker: HIS3
560	p424CYC yeast expression vector	Promoter: CYC1 \| Origin: 2micron \| Marker: TRP1
560	p424ADH yeast expression vector	Promoter: ADH1 \| Origin: 2micron \| Marker: TRP1
560	p424TEF yeast expression vector	Promoter: TEF1 \| Origin: 2micron \| Marker: TRP1
560	p425CYC yeast expression vector	Promoter: CYC1 \| Origin: 2micron \| Marker: LEU2
560	p425ADH yeast expression vector	Promoter: ADH1 \| Origin: 2micron \| Marker: LEU2
560	p425TEF yeast expression vector	Promoter: TEF1 \| Origin: 2micron \| Marker: LEU2
560	p425GAL-S yeast expression vector	Promoter: GAL1-S \| Origin: 2micron \| Marker: LEU2
560	p425GAL-L yeast expression vector	Promoter: GAL1-L \| Origin: 2micron \| Marker: LEU2
560	p426CYC yeast expression vector	Promoter: CYC1 \| Origin: 2micron \| Marker: URA3
560	p426TEF yeast expression vector	Promoter: TEF1 \| Origin: 2micron \| Marker: URA3
560	p426GAL-S yeast expression vector	Promoter: GAL1-S \| Origin: 2micron \| Marker: URA3
560	p426GAL-L yeast expression vector	Promoter: GAL1-L \| Origin: 2micron \| Marker: URA3

KickStart expression system vectors

Applications

Expression of recombinant proteins in S. cerevisiae
Expression of membrane proteins and secreted proteins

Benefits

Protein expression in rich medium (YPD)
Self-inducing system, no addition of inducer required
Protease deficient strain for higher protein yields

Price (€):	Product name	Description
560	pKS1 tagless expression vector	Promoter: ADH2 \| Origin: 2micron \| Marker: KanMX (G418 selection) \| Tag: -

Yeast expression strains

Applications

Expression of recombinant proteins in yeast
Intracellular and secreted expression
Protease deficient strains

Price (€):	Product name	Description
390	DSY-5 yeast expression strain	Protease deficient yeast strain (Pep4/ Prb1)
390	DSY-6 yeast expression strain	Protease deficient yeast strain (Pep4/ Prb1/Prc1)

Expression in mammalian cells

Expression system for protein expression in mammalian cells

Expression of recombinant proteins in mammalian cells
Transient and stable expression
Follow-up co-immunoprecipitation studies after DUALhybrid (yeast two-hybrid), DUALmembrane and DUALhunter screens

Mammalian expression vectors

Applications

Expression of recombinant proteins in mammalian cells
Transient and stable expression
Follow-up co-immunoprecipitation studies after DUALhybrid (yeast two-hybrid), DUALmembrane and DUALhunter screens

Price (€):	Product name	Description
560	pHA-MEX mammalian expression vector	CMV Promoter \| HA tag (N-terminal) \| transient and stable expression vector
560	pMEX-HA mammalian expression vector	CMV Promoter \| HA tag (C-terminal) \| transient and stable expression vector

Products

Suppliers

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Yeast two-Hybrid | cDNA Library Construction

Protein expression

Expression in E. coli

QuickLyse pEAS1-a expression vector

Applications

Benefits

Applications

Benefits

Expression in yeast

Applications

Benefits

Applications

Applications

Benefits

Applications

Expression in mammalian cells

Applications

Products

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