25-OH Vitamin D Total, ELISA, 96 tests and ELISA 25-Hydroxyvitamin D TotaL


Novel and unique ELISA 25-Hydroxyvitamin D TotaL

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The  25-Hydroxyvitamin D Total ELISA is powered by unique  monoclonal antibodies that express  100 % reactivity against 25 OH Vitamin D3  and 83% reactivity against 25OH Vitamin D2.
All In One technology. No extraction step, displacement solution directly into the well. Results in less than 4 hours. Calibrated against ID-LC/MS-MS (reference method).  Bar codes on all components.

Cat#

Description

Kit

Quantity

Price

GDMS 1971

25OH Vitamin D Total ELISA

96T

1-10

€ 180

GDMS 1971

25OH Vitamin D Total ELISA

96T

11-50

€ 155

GDMS 1971

25OH Vitamin D Total ELISA

96T

> 50

€ 145

 

Technical spec.:

Catalog # GDMS 1971
Format ELISA MT
Total Incubation Time < 4 hours
Size 96 tests
Sample Type Serum
Sample Volume 50 µL
Controls 2 levels
Range 0-180 ng/mL
Sensitivity 1,4 ng/mL
Protocol steps 120min./30 min./15 min
Wavelength 450 nm

 

Protocol comparison:

GENTAUR

Others

Pretreatment

None

25 µL of sample, calibrator and control + 1mL
of Biotin Solution into a glass or
polypropylene tube

Vortex

Calibrators/Controls/Samples

50 µL

Add 200 µL of diluted sample in the
appropriate wells

Incubation Buffer

150 µL

Incubation

Incubate 2 hours at room temperature with
shaking

Incubate 2 hours at room temperature

Washing

Aspirate and wash 3 times

Aspirate and wash 3 times

Diluted conjugate

200 µL

200 µL

Incubation

Incubate 30 min. at room temperature with
shaking

Incubate 30 min. at room temperature

Washing

Aspirate and wash 3 times

Aspirate and wash 3 times

Ready to Use TMB

100 µL

200 µL

Incubation

Incubate 15 min. at room temperature with
shaking

Incubate 30 min. at room temperature

Stop Solution

100 µL

100 µL

Total turnaround time

2h45 min.

3h30 min.

 

 

 

 

25-OH Vitamin D Total, ELISA, 96 tests

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The Gentaur 25-OH Vitamin D Total ELISA is a solid phase Enzyme Linked Immunosorbent Assay performed on microtiterplates. During a first 2 hours incubation step, at room temperature, total 25-OH Vitamin D (D2 and D3) present in calibrators, controls and samples is dissociated from binding serum proteins to fix on binding sites of a specific monoclonal antibody. After 1 washing step, a fixed amount of 25-OH Vitamin D-labelled with biotin in presence of horseradish peroxidise (HRP), compete with unlabelled 25-OH Vitamin D2 and 25-OH Vitamin D3 present on the binding sites of the specific monoclonal antibody. After a 30 minutes incubation at room temperature, the microtiterplate is washed to stop the competition reaction. The Chromogenic solution (TMB) is added and incubated for 15 minutes. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is inversely proportional to the total 25-OH Vitamin D (D2 and D3) concentration. A calibration curve is plotted and the total 25-OH Vitamin D (D2 and D3) concentrations of the samples are determined by dose interpolation from the calibration curve.

 

Price: 250 €
 

 

Technical spec.:

Catalog # KAP1971
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum
Sample Volume 50 µL
Controls 2 levels
Range 0-180 ng/mL
Sensitivity 1,4 ng/mL
Incubation 120min./30 min./15 min
Shelf Life (weeks) 32

 

Download:

Download "Instructions for Use"