Olaquindox metabolite (MQCA) ELISA test kit

Overview
The quinoline B alcohol is a quinoxaline -1,4 - dioxo of a physical class of compounds , has been as pro- born long agent wide pan should be used in the livestock , poultry , aquatic raise colonization . Because olaquindox have mutagenic to humans and animals , the EU in 1997 olaquindox . China restricted 3 5 kg pig ban ended in poultry , aquaculture use. The quinoline B alcohol residue standard diagram was 3 - A group quinoxaline Lin -2 - carboxylic acid (3 - m Eth y l- q UI n o x al i ne - 2 - c ARB o x y LIC a c i d , M q CA) . Our regulations set MQCA maximum residue remain limited to the pig liver dirty 50 μ g / kg pig muscle 4 μ g / kg . At present , at home and abroad has not been detection olaquindox residues markers MQCA residues. This kit is simple , fast , accurate and high sensitivity applies to muscle meat (pig , chicken , fish , shrimp ) , liver (pork, chicken) Olaquindox residues marker 3 - methyl quinoxaline -2 - carboxylic acid (MQC A) of the residue testing.

Principle
This kit according to the principle of the antigen-antibody specific immune response, using the indirect competitive ELISA was used to detect the sample the olaquindox metabolites material (MQCA) residual stay . Detection , standard solution or sample MQCA microtiter strips pre-coated with coupling antigen competing of contention MQCA antibodies. washed to remove unbound antibody . Add enzyme-labeled antibody , the with the the adsorbed on the plate . Washed to remove unbound enzyme-labeled antibody, substrate was added remarkable color (to blue ), most after adding the Stop Solution in 4 0 n m at measuring the absorbance values. Absorption of light value of the size of the sample in MQCA concentration negatively correlated.

Kit technical parameters :
Muscle meat (pork, chicken, fish, shrimp) 2 p p b
Liver ( pork, chicken) 5 ppb
The limit of quantification:
Muscle meat (pork, chicken, fish, shrimp) 4 p p b
Liver ( pigs, chickens) 5p p b

Cross-reactive response rate:
3 - methyl quinoxaline -2 - carboxylic acid ( MQC A ) ............... 100
Quinoxaline -2 - carboxylic acid ( QCA ) ............................... 1.0%
Off dioxane the quinoline B alcohol ........................................ < 1.0%
Quinoline ene ketone ............................................... .... < 1.0%
Off dioxide quinoline ene ketone ........................ < 1.0%
Methacholine quinoline ............................................ < 1.0%
Recoveries : of MQCA in various tissues average recovery 6 0 % to 2 %
Precision: the kit board, the board coefficient of variation <20%

Price: 850 €