Lentivirus and Retrovirus Packaging


Lentivirus Packaging

Lentiviral / retroviral vectors are one of the most efficient methods of gene delivery to mammalian cells both in vitro and in vivo. Through years of experience with lentiviral and retroviral vectors, We have developed own proprietary packaging systems and efficient protocols for the rapid generation of pseudoviral particles.

We offer express lentiviral/retrovirus packaging service to produce high-quality, high-titer virus particles using your viral construct with turnaround time of 10 days. Save your time and receive ready-to-transduce viral particles.

Features of our virus packaging services
  • Production of virus in a state-of-the art BSL-2 facility with robust quality control in accordance with NIH Biosafety Level 2 criteria
  • Flexibility of virus production scales to meet your research needs
  • Accurate Virus titers as determined by qPCR to measure infectious units per ml ( ifus/ml)
 
Note:
  • Depending on level of titer requested, please provide 10-40 μg of endotoxin-free lentivector or retrovector plasmid DNA
  • All custom virus production services should come with information on the plasmids which have to be packaged.
  • Vector must be able to produce virus (no internal poly A signal, no toxic genes, no unusual 2nd structure, or 5' to 3' LTR size over 8kb.

 

Service  Name Catalog # Spec. Price
Regular Titer ASC-7011 >10^7 IFU/ml, 20 ul 900 EUR
High Titer ASC-7012 >10^8 IFU/ml, 20 ul 1100 EUR
Ultra High Titer ASC-7013 >10^9 IFU/ml, 20 ul 1400 EUR

 

Retrovirus Packaging

Retroviral vectors are one of the efficient methods of gene delivery to mammalian cells both in vitro and in vivo. Through years of experience with lentiviral and retroviral vectors, We have developed own proprietary packaging systems and efficient protocols for the rapid generation of pseudoviral particles.

We offer express Retrovirus packaging service to produce high-quality, high-titer virus particles using your viral construct with turnaround time of 10 days. Save your time, receive ready-to-transduce viral particles.

 
Features of Our virus packaging services
  • Production of virus in a state-of-the art BSL-2 facility with robust quality control in accordance with NIH Biosafety Level 2 criteria
  • Flexibility of virus production scales to meet your research needs
  • Accurate Virus titers as determined by qPCR to measure infectious units per ml ( ifus/ml)
 
Note:
  • Depending on level of titer requested, please provide 10-40 μg of endotoxin-free lentivector or retrovector plasmid DNA
  • All custom virus production services should come with information on the plasmids which have to be packaged.
  • Vector must be able to produce virus (no internal poly A signal, no toxic genes, no unusual 2nd structure, or 5' to 3' LTR size over 8kb.)

 

 

Service  Name Catalog # Spec. Price
Regular Titer ASC-7021 >10^6 IFU/ml, 20 ul 1100 EUR
High Titer ASC-7022 >10^7 IFU/ml, 20 ul 1400 EUR

 

DNA Cloning Service

With decades of experience our Molecular Biology Team has developed an exceptional expertise in anything DNA-related. We  can help you develop the best strategy to tackle your cloning projects and find solutions to all your technical problems. 

 

Some examples of our services:

  • Cloning of amplified DNA fragments
  • Gene targeting vectors for homologous recombination
  • Bacterial Artificial Chromosome (BAC) Recombineering
  • RNAi and inducible vectors
  • Site-specific mutagenesis
  • Tagging of genes
  • De novo gene synthesis
Service  Name Catalog # Spec. Price
DNA Cloning Service ASC-7002 Gene expression vector 900 EUR/Step