ECOS™ Blue Competent Cells [XL1-Blue]
Strain: XL1-Blue

Description

XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.
Genotype

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F'proAB lacIqZDM15 Tn10 (TetR)]
Features

  • A strain modified to be ideal for blue/white screening
  • Tetracycline resistant
Applications

  • Routine cloning application
  • Subcloning
  • cDNA library
  • Blue/white screening
Efficiency

>5 x 107 cfu/ μg
Recommended for:
  • Routine cloning and subcloning
  • Blue/white screening
>2 x 108 cfu/μg
Recommended for:
Recommended for:
  • Routine cloning and subcloning
  • Blue/white screening
>5 x 108 cfu/ μg
Recommended for:
  • Routine cloning and subcloning
  • Blue/white screening
  • cDNA library
Specification
Store at

 

# FYE107-10VL (10 preps)
Efficiency >5 x 107 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
# FYE107-80VL (80 preps)
Efficiency >5 x 107 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
# FYE108-10VL (10 preps)
Efficiency >2 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
# FYE108-80VL (80 preps)
Efficiency >2 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
# FYE109-10VL (10 preps)
Efficiency >2 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
# FYE109-80VL (80 preps)
Efficiency >5 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
# FYE107-96WL (96 preps)
Efficiency >5 x 107 cfu/μg
50 μl/well 96-wells
# FYE108-96WL (96 preps)
Efficiency >2 x 108 cfu/μg
50 μl/well 96-wells
# FYE109-96WL (96 preps)
Efficiency >5 x 108 cfu/μg
50 μl/well 96-wells
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