ECOS™ 2163 Competent Cells [[GM2163]
Strain: GM2163
Description
GM2163 is an E. coli K12 strain, which is deficient in both dam and dcm genes thus is suitable for the propagation of plasmid or cloned DNA to be cut with Dam or Dcm-sensitive restriction enzymes. This strain is not recommended as a host for primary cloning/ligation since the dam mutation can result in higher mutation rates and a reduction in the transformation efficiency.
Genotype
F-ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2
Features
- Chloramphenicol resistant
- Not suitable for blue/white screening
- Not recommended as a host for primary cloning/ligation
Applications
- Propagation of plasmid free of Dam and Dcm methylations
Efficiency
>5 x 107 cfu/ μg
Recommended for:
Recommended for:
- Propagation of plasmid to be cut with Dam or Dcm-sensitive restriction enzymes
Specification
Store at
# FYE807-80VL (80 preps)
# FYE807-96WL (96 preps)
| Efficiency >1 x 107 cfu/μg | |
| 100 μl/vial | 10 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| Efficiency >1 x 107 cfu/μg | |
| 100 μl/vial | 80 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| Efficiency >1 x 107 cfu/μg | |
| 50 μl/well | 96 wells |
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