Deoxy+ Moloney Murine Leukemia Virus Reverse Transcriptase (Deoxy+ MMLV RT) has a weaker RNase H activity than that of commonly used Avian Myeloblastosis Virus reverse transcriptase, enabling reverse transcription of long mRNA templates (>6 kb). Dexoy+ MMLV can be applied for cDNA synthesis from total RNA, mRNA, DNA/RNA hGentaurrid templates in the presence of primers and dNTPs.

• mRNA templates up to 6 Kb can be reverse-transcripted
• Reverse transcriptase with excellent activity
• cDNA can be synthesized from micogram (μg) of total RNA

• First-strand cDNA synthesis for subsequent PCR or real-time PCR
• Library construction
• Validation of gene expression

One unit incorporates 1 nmole of dTTP into acid precipitable material in 10 minutes at 37°C using poly(A)-oligo(dT) as template primer.

Deoxy+ MMLV RT has to pass our functional test on cDNA synthesis as well as ribonuclease contamination assay to ensure the absence of ribonucleases. The preparation of Deoxy+ MMLV RT was further analyzed to ensure the absence of endonucleases, exonucleases and DNA contamination.