Genomic DNA Mini Kit (Blood/Cultured Cells)
Description

The Genomic DNA Kit (Blood/Cultured Cells) provides an efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungi. Chaotropic salt is used to lyse cells and degrade proteins, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 6 μg from 200 μl of whole human blood and up to 50 μg of DNA from 200 μl of buffy coat. Purified DNA, with approximately 20-30 Kb, is suitable for use in PCR or other enzymatic reactions.
Features

  • Sample : 
    up to 300 µl of whole blood
    up to 200 µl of frozen blood
    up to 200 µl of buffy coat
    cultured cells (up to 1 x 107) 
    bacterial cells (up to 1 x 109) 
    fungal cells (up to 5 x 107)

  • Format :
    spin column (centrifuge)

  • Yield :
    5-30 μg Operation time: within 30 minutes
Applications

PCR, AFLP/PADP, RFLP, Southern blotting, real-time PCR

Quality Control

The quality of the Genomic DNA Kit (Blood/Cultured Cells) is tested on a lot-to-lot basis by isolating genomic DNA from 200 μl of whole human blood. The purified DNA (4-6 μg with an A260 / A280 ratio of 1.6 - 1.8) is quantified with a spectrophotometer and checked by electrophoresis.

Protocol

  

Specification
Store at

 

# FYG101-100P (100 preps)
GT Buffer 30 ml
GB Buffer 40 ml
W1 Buffer 45 ml
Wash Buffer 25 ml
Elution Buffer 30 ml
RBC Lysis Buffer 135 ml
GD Column (50 mg/ml) 100 pcs
2 ml Collection Tube 200 pcs
# FYG101-300P (300 preps)
GT Buffer 75 ml
GB Buffer 100 ml
W1 Buffer 130 ml
Wash Buffer 50 ml
Elution Buffer 75 ml
RBC Lysis Buffer 405 ml
GD Column (50 mg/ml) 300 pcs
2 ml Collection Tube 600 pcs

 

Store at

 

RNase A
# FYG106-50UL
RNase A (50 mg/ml) 50 μl
# FYG106-130UL
RNase A (50 mg/ml) 130 μl
# FYG106-200UL
RNase A (50 mg/ml) 200 μl
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