Thermostable ß-Agarase is an enzyme which hydrolyzes ß-1,4 linkages in agarose to produce neoagaro-oligosaccharides. Agarose digested by Thermostable ß-Agarase does not gelate again, therefore, nucleic acids can be recovered from agarose gels. Thermostable ß-Agarase has a higher thermostability and stronger hydrolyzing activity than conventional Thermostable ß-Agarase. Furthermore, a simple protocol allows for quick DNA and RNA purification. This enzyme is particularly suitable for purification of intact large DNA.
Purification of DNA for direct use in cloning, restriction digestion and sequencing etc.
Unit definition one unit of Thermostable β-Agarase produces reducing sugar equivalent to 1 μmol of D-galactose per minute at 60°C.
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| Thermostable β-Agarase (1 U/μl) | 30 μl |
| Thermostable β-Agarase (1 U/μl) | 100 μl |