Viral Nucleic Acid Extraction Kit (DNA/RNA) is specially designed for purification of viral RNA or DNA from cell-free samples. With extraction method, DNA/RNA viruses are lysed quickly and efficiently by lysis buffer which is a highly concentrated solution of chaotropic salt. Lysis buffer and ethanol create appropriate conditions for binding of nucleic acids to the glass fiber matrix of the blood viral DNA/RNA binding column. Contaminations like salts, metabolites and soluble macromolecular cellular components are removed in washing steps. The nucleic acids can be eluted in low salt buffer or water and are ready-to-use in subsequent reactions.
The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assay. This protocol is recommended for parallel purification of viral RNA including HCV, HIV, and HTLV and viral DNA including HBV and CMV.
- Sample :
serum, plasma, cell-culture supernatants, other cell-free body fluids - Sample size :
200 μl - Virus type :
retroviruses, influenza, enteroviruses, DNA viruses, etc.
- PCR
- Real-time PCR
- RT-PCR
- Automated fluorescent DNA sequencing
- Enzymatic reactions
| VB Lysis Buffer | 30 ml |
| AD Buffer¹ | 4 ml |
| WI Buffer | 30 ml |
| Wash Buffer² | 12.5 ml |
| RNase-free Water | 6 ml |
| VB Column | 50 pcs |
| 2 ml Collection Tube | 100 pcs |
¹ Add 30 ml of absolute ethanol to the AD Buffer prior to initial use ² Add 50 ml of absolute ethanol to the Wash Buffer prior to initial use |
|
| VB Lysis Buffer | 60 ml |
| AD Buffer¹ | 8 ml |
| WI Buffer | 50 ml |
| Wash Buffer² | 25 ml |
| RNase-free Water | 6 ml |
| VB Column | 100 pcs |
| 2 ml Collection Tube | 200 pcs |
¹ Add 60 ml of absolute ethanol to the AD Buffer prior to initial use ² Add 100 ml of absolute ethanol to the Wash Buffer prior to initial use |
|
| VB Lysis Buffer | 130 ml |
| AD Buffer¹ | 24 ml |
| WI Buffer | 130 ml |
| Wash Buffer² | 50 ml |
| RNase-free Water | 30 ml |
| VB Column | 300 pcs |
| 2 ml Collection Tube | 600 pcs |
¹ Add 180 ml of absolute ethanol to the AD Buffer prior to initial use ² Add 200 ml of absolute ethanol to the Wash Buffer prior to initial use |
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