HiYield Gel/PCR DNA Fragment Extraction Kit
Description

The HiYield Gel/PCR DNA Fragment Extraction Kit is designed to recover or concentrate DNA fragments (100 bp→10 Kb) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer or TE. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. The entire procedure can be completed in 20 minutes.
Features

  • Recovery :
    up to 90% (gel extraction) up to 95% (PCR cleanup)

  • Operation time :
    20 minutes for gel extraction 15 minutes for PCR cleanup
Applications

Fluorescent/radioactive sequencing, PCR, restriction enzyme digestion, DNA labeling and ligation
Quality Control

The quality of the HiYield Gel/PCR DNA Fragment Extraction Kit is tested on a lot-to-lot basis by isolating DNA fragments of various sizes from either aqueous solutions or agarose gels. The purified DNA is checked by electrophoresis.
Results

 

Specification
Store at

 

# FYG201-100P (100 preps)
DF Buffer 80 ml
Wash Buffer 25 ml
W1 Buffer 45 ml
Elution Buffer 6 ml
DF Column 100 pcs
2 ml Collection Tube 100 pcs
# FYG201-300P (300 preps)
DF Buffer 240 ml
Wash Buffer 50 ml
Wash Buffer 25 ml
W1 Buffer 130 ml
Elution Buffer 30 ml
DF Column 300 pcs
2 ml Collection Tube 300 pcs
# FYG202-10P (Maxi)
(10 preps)
DF Buffer 125 ml
Wash Buffer 25 ml
W1 Buffer 90 ml
Elution Buffer 12 ml
DM Column 10 pcs
# FYG202-25P (Maxi)
(25 preps)
DF Buffer 290 ml
Wash Buffer 75 ml
W1 Buffer 210 ml
Elution Buffer 30 ml
DM Column 25 pcs
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