The Total RNA Extraction Mini Kit (Animal Tissues) is designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required.
- Sample :
up to 25 mg of tissues
up to 25 mg of paraffin-embedded tissues - Format :
spin column (centrifuge) - Yield :
5-30 μg - Operation time :
within 30 minutes
RT-PCR, Northern blotting, primer extension, mRNA selection, cDNA synthesis, RNase protection assay
The quality of the Total RNA Extraction Mini Kit (Animal Tissues) is tested on a lot-to-lot basis by isolating total RNA from a 25 mg animal tissue sample. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.
| RB Buffer | 30 ml |
| W1 Buffer | 30 ml |
| Wash Buffer | 12.5 ml |
| Rnase-Free water | 6 ml |
| RB Column | 50 pcs |
| Filter Column | 50 pcs |
| 2 ml Collection Plate | 100 pcs |
| Micropestle (RNase-free) | 50 pcs |
| RB Buffer | 60 ml |
| W1 Buffer | 50 ml |
| Wash Buffer | 25 ml |
| Rnase-Free water | 15 ml |
| RB Column | 50 pcs |
| Filter Column | 100 pcs |
| 2 ml Collection Plate | 200 pcs |
| Micropestle (RNase-free) | 100 pcs |
| RB Buffer | 130 ml |
| W1 Buffer | 130 ml |
| Wash Buffer | 50 ml × 2 |
| RNase-Free water | 30 ml |
| RB Column | 300 pcs |
| Filter Column | 300 pcs |
| 2 ml Collection Plate | 600 pcs |
| Micropestle (RNase-free) | 300 pcs |
