The Fast Ion Plasmid Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50-100 ml / 100-250 ml of cultured bacterial cells. Modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the column and contaminants are removed with a wash Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in 2 / 2.5 hours without ultracentrifuges, HPLC or other toxic compound.

• Sample :
  50 ml of cultured bacterial cells for high-copy number plasmids
  100 ml of cultured bacterial cells for high-copy number plasmids
  250 ml of cultured bacterial cells for low-copy number plasmids


• Yield :
  up to 250/500 μg of plasmid


• DNA Format :
  gravity flow

Transfection, sequencing, ligation, PCR, in vitro transcription, microinjection, restriction enzyme digestion, gene gun

The quality of the Fast Ion Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 50 ml overnight E. coli (DH5a) culture, containing plasmid pBluescript (A₆₀₀ > 2 U/ml). More than 120 μg of plasmid DNA is quantified with a spectrophotometer. The purified plasmid (1 μg) is used in EcoR I digestion, and checked by electrophoresis.