The Genomic DNA Mini Kit (Animal Tissues) is designed specifically for total DNA purification, including genomic DNA purification, mitochondrial DNA purification and virus DNA purification from a variety of animal tissue, paraffin-embedded tissue, buccal swab and amniotic fluid. The provided micropestle can efficiently homogenize tissue samples to shorten the time in the lysis step. Proteinase K and chaotropic salt are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fiber matrix of the spin column. Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer or TE.
- Sample :
up to 30 mg of tissue,
up to 25 mg of paraffin-embedded tissue,
buccal swab and up to 15 ml of amniotic fluid - Format :
spin column (centrifuge) - Yield :
up to 50 μg
PCR, AFLP/PADP, RFLP, Southern blotting, real-time PCR
The quality of the Genomic DNA Mini Kit (Animal Tissues) is tested on a lot-to-lot basis by isolating genomic DNA from a 20 mg mouse liver sample. The Purified DNA (more than 10 μg with an A260 / A280 ratio of 1.8 – 2.0) is quantified with a spectrophotometer and checked by electrophoresis.
| GT Buffer | 30 ml |
| GBT Buffer | 40 ml |
| W1 Buffer | 45 ml |
| Wash Buffer | 25 ml |
| Elution Buffer | 30 ml |
| Proteinase K | 11 mg |
| GD Column | 50 pcs |
| 2 ml Collection Tube | 100 pcs |
| Micropestle | 50 pcs |
| GT Buffer | 60 ml |
| GBT Buffer | 60 ml |
| W1 Buffer | 45 ml |
| Wash Buffer | 25 ml |
| Elution Buffer | 30 ml |
| Proteinase K | 11 mg × 2 |
| GD Column | 100 pcs |
| 2 ml Collection Tube | 200 pcs |
| Micropestle | 100 pcs |
| GT Buffer | 155 ml |
| GBT Buffer | 155 ml |
| W1 Buffer | 130 ml |
| Wash Buffer | 50 ml |
| Elution Buffer | 75 ml |
| Proteinase K | 65 mg |
| GD Column | 300 pcs |
| 2 ml Collection Tube | 600 pcs |
| Micropestle | 300 pcs |
