RNA Pure Kit
Description

The RNA Pure Kit is designed to purify or concentrate RNA products which have been previously isolated using a variety of RNA isolation methods. The unique RNA Pure Buffer ensures easy binding of RNA to the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the ultra pure RNA is eluted by RNase-free water or TE (RNase-free). Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Features

  • Sample : up to 100 μl of RNA products
  • Format : spin column (centrifuge)
  • Recovery : up to 90%
  • Operation time : within 20 minutes
Applications

  • RT-PCR
  • Northern blotting
  • PCR
  • Fluorescent or radioactive sequencing
  • Restriction enzyme digestion
  • DNA labeling and ligation
Quality Control

The quality of the RNA Pure Kit is tested on a lot-to-lot basis by purifying RNA of various sizes from aqueous solutions. The purified RNA is checked by electrophoresis.

Protocol

Specification
Store at

 

# FYG602-50P (50 preps)
RNA Pure Buffer 30 ml
Wash Buffer* 12.5 ml
RNase free Water 6 ml
PR Column 50 pcs
2 ml Collection Tube 50 pcs

*Add 50 ml of absolute ethanol to the Wash Buffer prior to initial use

 

# FYG602-100P (100 preps)
RNA Pure Buffer 80 ml
Wash Buffer* 25 ml
RNase free Water 6 ml
PR Column 100 pcs
2 ml Collection Tube 100 pcs

*Add 50 ml of absolute ethanol to the Wash Buffer prior to initial use

 

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