The Genomic DNA Kit (Plant Tissues) provides a quick and easy method for purifying total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from plant tissues. Samples are disrupted by grinding in liquid nitrogen followed by lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The procedure does not require phenol extraction or alcohol precipitation, and can be completed in less than 1 hour.
- Sample :
up to 100 mg (fresh plant tissues)
up to 25 mg (dry plant tissues) - Format :
spin column (centrifuge) - Yield :
up to 50 μg
PCR, AFLP/PADP, RFLP, Southern blotting, real-time PCR
The quality of the Genomic DNA Kit (Plant Tissues) is tested on a lot-to-lot basis by isolating genomic DNA from 50 mg young leaf samples. More than 10 μg of genomic DNA is quantified with a spectrophotometer and checked by electrophoresis.
(100 preps)
| GP1 Buffer | 50 ml |
| GPX1 Buffer | 50 ml |
| GP2 Buffer | 15 ml |
| GP3 Buffer | 30 ml |
| W1 Buffer | 45 ml |
| Wash Buffer | 25 ml |
| Elution Buffer | 30 ml |
| RNase A (10 mg/ml) | 550 μl |
| Filter Column | 300 pcs |
| GD Column | 300 pcs |
| 2 ml Collection Tube | 600 pcs |
(10 preps)
| GP1 Buffer | 50 ml |
| GPX1 Buffer | 50 ml |
| GP2 Buffer | 15 ml |
| GP3 Buffer | 30 ml |
| W1 Buffer | 45 ml |
| Wash Buffer | 25 ml |
| Elution Buffer | 30 ml |
| RNase A (10 mg/ml) | 550 μl |
| Filter Column | 10 pcs |
| GD Column | 10 pcs |
(25 preps)
| GP1 Buffer | 125 ml |
| GPX1 Buffer | 125 ml |
| GP2 Buffer | 30 ml |
| GP3 Buffer | 70 ml |
| W1 Buffer | 130 ml |
| Wash Buffer | 50 ml |
| Elution Buffer | 60 ml |
| RNase A (10 mg/ml) | 650 μl × 2 |
| Filter Column | 20 pcs |
| GD Column | 20 pcs |
