The 96-Well Plasmid Kit is designed for high-throughput plasmid DNA purification from 1-2 ml of cultured bacterial cells. Modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of each well of the plate. Contaminants are removed with a wash buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt elution buffer or TE. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 1 hour.

• Operation :
  centrifuge/vacuum manifold


• Yield :
  5-10 μg for high-copy plasmid                                                               0.5-5 μg for low-copy plasmid


• Operation time :
  within 60 minutes

• Fluorescent/radioactive sequencing
• Restriction enzyme digestion
• Library screening, ligation
• Transformation and PCR

The quality of the 96-Well Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 1.5 ml overnight E. coli (DH5a) culture, containing plasmid pBluescript (A₆₀₀ > 2 U/ml). More than 8 μg of plasmid DNA can be quantified with a spectrophotometer. The purified plasmid (1 μg) is used in EcoR I digestion, and checked by electrophoresis.