The Small DNA Fragment Extraction Kit was designed to recover or concentrate DNA fragments (50-200 bp) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer or TE. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are up to 90% for Gel Extraction and up to 95% for PCR Clean up. The entire procedure can be completed in 20 minutes.

The Large DNA Fragment Extraction Kit is designed to recover or concentrate DNA fragments (> 8 Kb) from agarose gel in 4 easy steps. Salts and enzymes can be effectively removed from the reaction mixture without phenol extraction. Typically, recoveries are up to 85% for Gel Extraction. The entire procedure can be completed in 45 minutes.

Small DNA Fragment Extraction Kit:

• Recovery : up to 95%
• Operation time : within 20 minutes

 

Large DNA Fragment Extraction Kit

• Recovery : up to 85%
• Operation time : within 45 minutes

Fluorescent/radioactive, sequencing, PCR, restriction enzyme digestion, DNA labeling and ligation

The quality of the Small/Large DNA Fragment Extraction Kit is tested on a lot-to-lot basis by isolating DNA fragments of various sizes from either aqueous solutions or agarose gels. The purified DNA is checked by electrophoresis.