JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics. 

This product can become a powerful cloning tool when it is used in combination with Gentaur’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

e14^-(McrA^-) recA1 endA1 gyrA96 thi-1 hsdR17(rK^- mK⁺) supE44 relA1 D(lac-proAB) [F’ traD36 proAB lacIqZDM15]

• Rapid growing strain
• Carries F' episome

• Preparation of ssDNA
• Construction of gene library
• Subcloning

• High efficiency cloning
• Cloning low abundance transcripts
• General cloning
• Gene library

• Super high efficiency cloning
• Cloning lowest abundance transcripts
• Gene library

• Subcloning
• General cloning