The 96-Well Genomic DNA Extraction Kit is designed for high-throughput purification of total DNA (including genomic, mitochondrial and viral DNA) from a variety of samples. Proteinase K and chaotropic salt are used to lyse cells and degrade protein, allowing DNA in the chaotropic salt to be easily bound by the glass fiber matrix of each well of the plate. Once contaminant have been removed, the purified DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation. The kits can be used for manual filtration or with robotic handling systems and the purified DNA with approximately 20-30 Kb is suitable for use in PCR or other enzymatic reactions.
- Sample :
up to 200 µl of blood
up to 20 mg of tissues
cultured cells (up to 1 x 107)
bacterial cells (up to 1 x 109)
fungus cells (up to 5 x 107)
hair, blood spot
up to 200 µl of semen - Format :
96-well plate - Yield :
up to 30 μg/well
PCR, AFLP/PADP, RFLP, Southern blotting, real-time PCR
The quality of the 96-Well Genomic DNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μl of fresh whole human blood. The purified DNA (4-6 μg with an A260 / A280 ratio of 1.6 - 1.8) is quantified with a spectrophotometer and checked by electrophoresis.
| GT Buffer | 120 ml |
| GBT Buffer | 120 ml |
| W1 Buffer | 130 ml |
| Wash Buffer | 50 ml |
| Elution Buffer | 60 ml |
| Proteinase K | 45 mg × 2 |
| DNA Binding Plate | 4 pcs |
| 0.35 ml Collection Plate | 4 pcs |
| Adhesive Film | 8 pcs |
| GT Buffer | 240 ml |
| GBT Buffer | 240 ml |
| W1 Buffer | 130 ml × 3 |
| Wash Buffer | 50 ml |
| Elution Buffer | 120 ml |
| Proteinase K | 45 mg × 2 |
| DNA Binding Plate | 10 pcs |
| 0.35 ml Collection Plate | 10 pcs |
| Adhesive Film | 20 pcs |
