Gentaur's Products: Lyophilized Exosomes, Proteomic Kits, Exosome Immunocapture and Isolation Tools
Exosomes are small endosome-derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome relase occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner so that both amount and molecular composition of released exosomes depend on the state of a parent cell. This is one of major premises of employing exosomes for research and diagnostic purposes. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes incorporate a wide range of membranes and cytosolic proteins involved in many cellular functions and reflecting both their endosomal origin and a protein fingerprint unique for parent cell type and condition.
Exosomes shuttle also RNA molecules, mRNAs and miRNAs, both classes proved to be functional in target cell, being translated or modulating gene silencing respectively. Overall, exosome molecular composition distinguishes resting from activated, healthy from diseased cell.
ExoTESTtm is a novel, reliable, versatile and easy to use double sandwich ELISA assay for quantitative and qualitative analysis of exosomes.
The ExoTESTtm platform consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. We offer different types of tools (plates or beads) to capture exosomes from specific biological fluids (complex fluids or cell culture supernatants). Tools for exosome capture from complex fluids (plasma, urine, etc) are further distinguished in order to enable pan-exosome or exosome sub-populations capture. Quantification and characterization of exosomal proteins is subsequently performed by using appropriate detection antibodies against exosome associated antigens that can be either common to all exosomes or cell-type or cell-condition specific. ExoTESTtm enables detection of multiple exosome associated antigens indicative of type and condition of the parent cell, providing thus a platform for assessment of cell state and function. ExoTESTtm provides some immediate readouts, namely origin, quantity and molecular composition of isolated exosomes.
Specific ExoTESTtm kits allow easy and highly efficient RNA (mRNA or miRNA) extraction from the samples of interest.
HansaBioMed offers a wide range of products for exosome research which include the following categories:
- Exosome Immunocapture and Isolation tools (plates and beads)
- Exosome standards from characterized cell lines or human biofluids
- Exosomes Binding Antibodies for quantification and characterization
- Proteomic Kits for Exosomes quantification
- Total RNA Extraction Kits
- Customized products and services
Products:
Product Overview: Lyophilization is an ideal method for long-term storage of exosomes. It is proved to be stable upon a long-term storage at 4ºC and suitable as reference samples for multiple applications (FACS, WB, ELISA) and as calibration standards for quantitative comparison of different biological samples.
Moreover, lyophilized standards are easy to store, ship, and give excellent results with our a platform for proteomic analysis. Exosomes are purified from different biological sources (cell culture supernatant, human plasma, urine) with a combination of ultracentrifugation and microfiltration procedures for subsequent quantitative analysis and lyophilization.
Storage Information (lyophilized exosomes): Store at 4°C. Avoid repeated
Storage information (reconstituted product): Stored at -20°C for up to one month or at -80°C for up to 12 months. Strictly avoid repeated freeze-and-thaw cycles
Applications :
- Exosome quantification
- RNA and Proteins profiling
- RNA Extraction
Exosome Immunocapture and Isolation Tools
EXOTESTTM precoated plate for exosome capture. Transparent ELISA plate for colorim.
Proteomic Kits
EXOTESTTM basic kit for exosome capture.
Transparent plate for colorimetric readings with anti-CD63 primary antibody (for positive control detection only).
The primary Ab provided within the kit is meant for positive control.
Exosomes captured on the ELISA plate in such way can be further characterised by using the primary Antibody of interest or can be lysated in the same well and used for amplification of RNA sequences.
