Yeast Knock Out Collection
In 1996 S. cerevisiae became the first eukaryotic genome to be fully sequenced enabling the creation of the first molecular-barcoded, genome-wide yeast knockout collection (1, 2). The collection includes over 20,000 deletion strains corresponding to 5,916 genes (including 1,159 essential genes).
Each strain has a complete start-codon to stop-codon deletion of an open reading frame (ORF) that is flanked by two molecular barcodes. This collection has been used successfully in a number of screens and has been cited over 590 times (3).
Advantages of the collection include:
- Molecular barcodes allow high throughput parallel screening of entire genome
- Complete deletions that ensure no residual gene function
- Homozygous and heterozygous formats ready for screening
- Haploid collections can be crossed to cell based assay strains to expand screening options
Yeast Deletion Collection
| Cat# | Product Name | More information |
|---|---|---|
| TKY3500 | Yeast Knockout Collection Homozygous Diploid | |
| TKY3501 | Yeast Knockout Collection Heterozygous Diploid | |
| TKY3502 | Yeast Knockout Collection Haploid Mat-a | |
| TKY3503 | Yeast Knockout Collection Haploid Mat-alpha | |
| TKY3504 | Yeast Knockout Collection Essential Genes | |
| TKY0001 | Yeast Knockout Collection Parental Strain - S288C | |
| TKY0002 | Yeast Knockout Collection Parental Strain - BY4741 | |
| TKY0003 | Yeast Knockout Collection Parental Strain - BY4742 | |
| TKY0004 | Yeast Knockout Collection Parental Strain - BY4743 | |
| TKY1000 | Yeast Knockout Collection Homozygous Diploid Individual Strain | |
| TKY1001 | Yeast Knockout Collection Heterozygous Diploid Individual Strain | |
| TKY1002 | Yeast Knockout Collection Mat-a Haploid Individual Strain | |
| TKY1003 | Yeast Knockout Collection Mat-alpha Haploid individual strain |
