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Pooled Screening Deconvolution Service


Need help deconvoluting your pooled screen? Send us your genomic DNA samples for sequencing and analysis.
 

•Simplify your screen - Sequencing service including library preparation, next generation sequencing and data analysis
•Cost effective - Multiplex sequencing allows analysis of multiple samples in parallel
•Quick turnaround - 4-6 weeks from receipt of samples to delivery of data
•More data reproducibility - Optimized protocols and reagents for shRNA specific sequencing

In addition to providing the highest quality sensor-based shRNA pooled library, we also offer next generation sequencing analysis and deconvolution services. Provide us with the genomic DNA from the samples following your screen and we will provide the precise number of sequencing reads of each individual clone within a pooled shRNA sample.

 
Pooled shRNA screen Deconvolution Service is composed of the following steps:
  1. Your genomic DNA samples are evaluated for concentration and quality by Qubit® and agarose gel electrophoresis, respectively.  
  2. Integrated shRNA are amplified from the genomic DNA using mir30 specific primers while maintaining at least 1000X coverage of each shRNA.  
  3. Following column clean up and quantitation, low-cycle enrichment is performed using Illumina® adapted and indexed primers. Indexed-amplicon libraries are column purified, quantitated and evaluated by agarose gel electrophoresis.
  4. If necessary, magnetic bead purification or gel purification is performed to remove unamplified gDNA or primers.  
  5. Amplicon libraries are next diluted to a 10nM concentration followed by pooling of the individual samples.  
  6. The pooled sample is next denatured, diluted and loaded according to HiSeq® protocols and recommendations.  
  7. FASTQ data files for the 50bp sequencing run are produced.
  8. Sequence analysis is performed and counts are provided for each hairpin in each sample. 

 

Pooled screening libraries (plasmid or viral particle format)

Cat# Species Format Family Genes More information
TLH7002 Human Plasmid Phosphatases 255 Send request
TLH7102 Human Virus Phosphatases 255 Send request
TLH7004 Human Plasmid Ubiquitin ligases 146 Send request
TLH7104 Human Virus Ubiquitin ligases 146 Send request
TLH7032 Human Plasmid Cancer Metabolism 642 Send request
TLH7132 Human Virus Cancer Metabolism 642 Send request
TLH7051 Human Plasmid Kinases 951 Send request
TLH7151 Human Virus Kinases 951 Send request
TLH7034 Human Plasmid Druggable Genome 1971 Send request
TLH7134 Human Virus Druggable Genome 1971 Send request
TLH7033 Human Plasmid Whole Genome 18,455 Send request
TLH7133 Human Virus Whole Genome 18,455 Send request
TLH7009 Human Plasmid Epigenetics-related genes 406 Send request
TLH7109 Human Virus Epigenetics-related genes 406 Send request
TLM7010 Mouse Plasmid Epigenetics-related genes 311 Send request
TLM7110 Mouse Virus Epigenetics-related genes 311 Send request