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Sample Diluents
Improves Assays:
By accurately reading and quantitating high-titer samples. High-titer samples may overload the finite binding capacity of the coated ELISA plate surface, thus requiring dilution prior to testing in the assay.
Diluents Available:
  • ESD100 General-Purpose Sample Diluent - All ELISAs For dilution of most serum, ascites, cell-culture supernatants, and antigens into the functional range of the ELISA.
  • ESD200 Plasma Sample Diluent - Antigen-Down For dilution of plasma samples into the functional range of the antigen-down ELISA to inhibit complement and thrombin (clotting) activity during the assay.
  • ESD300 Picses Sample Diluent.
Directions:
Simply multiply the value determined from the curve by your dilution factor. For example, if you diluted your sample 1:2 (250uL sample into 250uL sample diluent) and this yielded an OD of 0.437, when read off the curve, the OD of 0.437 correlated to 200 pg/mL, you would simply multiply 200pg/mL by 2 (your dilution factor) yielding a value of 400pg/mL, meaning that the original starting sample had 400pg/mL of analyte present.
ESD100 General-Purpose Sample Diluent - All ELISAs
General-Purpose Sample Diluent is formulated for diluting goat, rabbit, mouse, and human sera, as well as cell culture media and mouse ascites fluid. ESD100 can also be used for dilution of mammalian and chicken serum samples in antigen-down ELISA formats. Hyperimmunized serum samples contain nonspecific IgG antibody that may interfere with the titration. Proprietary additives have been incorporated into this diluent that minimize nonspecific binding interactions of the non specific IgG in the samples, thus decreasing background noise. Dilutions of the antigen standard for use in sandwich ELISA formats have also been carried out using this diluent. ESD100 contains a buffered BSA protein base to assure a constant pH and solute environment favorable to antibody-antigen interactions. It also contains an antimicrobial agent to provide a solution free of bacterial contamination.

General-Purpose Sample Diluent (ESD100)
Catalog Number Size (mL) #Samples Diluted 1:2
using 250uL		 #Samples
diluted 1:10
using 450uL
ESD100-1 100 400 220X
ESD100-2 500 2000 1100
ESD200 Plasma Sample Diluent - Antigen-Down
Plasma Sample Diluent is formulated specifically for use with plasma samples being tested in an antigen-down ELISA format. This diluent contains proprietary additives in a buffered BSA protein base with which provide an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. Additional additives have been included to inhibit thrombin (clotting) and complement activity during the plasma sample incubation period. ESD200 also contains an antimicrobial agent to provide a solution free of bacterial contamination.

Plasma Sample Diluent - Antigen-Down (ESD200)
Catalog Number Size (mL) #Samples Diluted 1:2
using 250uL		 #Samples
diluted 1:10
using 450uL
ESD200-1 100 400 220
ESD200-2 500 2000 1100
ECD200-3 1000 4000 2200
ECD200-4 10000 40000 22200
ESD300 Nonmammilian Sample Diluent
Nonmammilian Sample Diluent contains proprietary additives in a buffered BSA protein base with which provide an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. ESD300 also contains an antimicrobial agent to provide a solution free of bacterial contamination.

Pisces Sample Diluent - (ESD300)
Catalog Number Size (mL) #Samples Diluted 1:2
using 250uL		 #Samples
diluted 1:10
using 450uL
ESD300-1 100 400 220
ESD300-2 500 2000 c1100
ECD300-3 1000 4000 2200
ECD300-4 10000 40000 22200