full screen background image

GENTAUR - Research solutions for the academic, biotechnology and pharmaceutical industries


aCella™ - cGMP 
Cyclic GMP Chemiluminescent Immunoassay Kit
Key Benefits:
  • Ultra-sensitive - Measures as little as < 1 fmol cGMP per sample.
  • Flexible - One kit for Extra- and Intra-Cellular cGMP quantification.
  • Convenient - Stable, 4 C liquid reagents.
  • Ready-to-Use - Complete kit with validated Cell Lysis Reagent Included.
Introduction to aCella - cGMP

Cyclic GMP is a critical and multifunctional secondary messenger present at levels typically 10-100 fold lower than cAMP in most tissues. Intracellular cGMP is formed by the action of the enzyme guanylate cyclase on GTP and degraded through phosphodiesterase hydrolysis. Guanylate cyclases (GC) are either soluble or membrane bound. Soluble GCs are nitric oxide responsive, whereas membrane bound GCs respond to hormones such as acetylcholine, insulin and oxytocin. Other chemicals like serotonin and histamine also cause an increase in cGMP levels. Cycle GMP regulates cellular composition through cGMP-dependent kinase, cGMP-dependent ion channels or transporters and through its hydrolytic degradation by phosphodiestererase.
Assay Principle

Gentaur's aCella – cGMP Chemiluminescent Immunoassay kit measures cGMP present in lysed cells, EDTA, plasma, urine, saliva and culture media samples. The kit is unique in that all samples and standards are diluted into an acidic Sample Diluent, which contain special additives and stabilizers, for cGMP measurement. Acidified samples of cGMP are stable and endogenous phosphodiesterases are inactived in the Sample Diluent. After an overnight incubation at 4 C, the plate is washed and the chemiluminescent substrate is added. The substrate generates light that is detected in a multi-label microtiter plate reader capable of reading luminescence.
Cyclic GMP Chemiluminescent Immunoassay Kit


Figure 1

Cyclic GMP Chemiluminescent Immunoassay Kit


Figure 2

 

Kit contents:
1. Coated White 96 Well Plates (Part# 90001): White plastic microtiter plate coated with donkey anti-donkey anti-sheep IgG. Store at 2-8 C upon arrival.
2. Cyclic GMP Standard (Part# 90002): 1 vial (70 uL) cyclic GMP at 640 pmol/mL in a special stabilizing solution. Store at 2-8 C upon arrival.
3. Cyclic GMP Antibody (Part# 90003): 1 bottle (3 mL) mouse monoclonal antibody specific for cyclic GMP. Store at 2-8 C upon arrival.
4. Cyclic GMP Conjugate Concentrate (Part# 90004): 1 vial (150 uL) cyclic GMP-peroxidase conjugate concentrate in a special stabilizing solution. Store at 2-8 C upon arrival.
5. Conjugate Diluent (Part# 90005): 1 bottle (3 mL) Diluent containing special stabilizers and additives. Store at 2-8 C upon arrival.
6. Sample Diluent (Part# 90006): 1 bottle (28 mL) diluent containing special stabilizers and additives. Ready to use. Store at 2-8 C upon arrival. WARNING: CAUSTIC.
7. Plate Primer (Part# 90007): 1 bottle (5 mL) neutralizing solution containing special stabilizers and additives. Store at 2-8 C upon arrival.
8. Acetic Anhydride (Part# 90008): 1 bottle (2 mL). Store at 2-8 C upon arrival. WARNING: Corrosive Lachrymator
9. Triethylamine (Part# 90009): 1 bottle (4 mL). Store at 2-8 C upon arrival. WARNING: Corrosive Lachrymator
10. Wash Buffer Concentrate (Part # 90010): 1 bottle (30 mL) 20x concentrate that must be diluted with deionized or distilled water. Store at 2-8 C upon arrival.
11. Substrate Solution A (Part # 90011): 1 bottle (6 mL). Upon arrival store at 2-8 C.
12. Substrate Solution B (Part # 90012): 1 bottle (6 mL). Upon arrival store at 2-8 C.
13. Plate Sealer (Part # 90013)

Product Catalog # Size*
aCella cGMP CLACGMP100-2 100