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Hydroxyl / Peroxynitrite Detection Kit ™
Fluorescent Hydroxyl (•OH) / Peroxynitrite (ONOO -) Detection Kit

Key Benefits:

Quenched Cell permeable dye.
One Step, No wash Homogenous assay.
Adaptable to High throughput assay platforms.
Can monitor multiple time points to follow real time kinetics.
Non-destructive cell based assay allows monitoring of additional parameters.
Applications - Fluorescent plate reader / Flow Cytometery / Fluorescent Microscopy.

Introduction:

A new novel probe, Hydroxyphenyl fluorescein (HPF), developed by Tetsuo Nagano et. al. (1), is a highly selective probe for the detection of highly Reactive Oxygen Species (hROS). It is a cell permeable highly sensitive fluorescent probe for hydroxyl radical (OH•), and peroxynitrite (ONOO-) detection. It has little reactivity towards other hROS such as: hypochlorite (-OCl), singlet oxygen (021), superoxide (02-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1.

Assay Principle:

Product	Catalog #	Contents	Size*
Hydroxyl / Peroxynitrite Detection Kit	FLHPF 100-2	HPF 1 vial	150