GENTAUR - Research solutions for the academic, biotechnology and pharmaceutical industries
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Fluoro IndoBlu
Fluoro IndoBlu Detection Kit
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| Key Benefits: |
- Highly effective and stable assay for cell proliferation.
- No need to wash out media from cell samples, just add the reagent directly to your experimental samples.
- Works for both cell viability and proliferation assay.
- Plate can be read in 30 minutes to 3 hours.
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| Introduction to Fluoro IndoBlu |
Gentaur introduces a Fluoro IndoBlu™ reagent for cell proliferation and viability assay. Fluoro IndoBlu™ is an oxidation/reduction- based reagent that functions as a cell viability indicator by quantitatively measuring the reducing power of living cells. Fluoro IndoBlu™ is a cell-permeable reagent that is blue in color, but non-fluorescent. When added to cells, the IndoBlu™ reagent is modified by the reducing environment of the viable cell, it turns red in color and becomes highly fluorescent. This change can be detected using fluorescence or absorbance measurements. Fluoro IndoBlu™ is detected at the Excitation = 530 nm and Emission = 590 nm/absorbance at 570nm.
Compared to the traditional cell proliferation assay, Gentaur’s novel in-cell-culture method is simple, fast and sensitive. The entire assay can be performed in a 96-well microtiter plate in 30 min and the best results are achieved in 3 hours. |
| Background |
Measurement of cell proliferation and viability is frequently used in clinical and experimental immunology as means of assessing cell activation in response to diseases, infections and environmental stimulations. Fluoro IndoBlu™ assay can be used for the measurement of cell proliferation in response to antigens, cytokines, growth factors, and mitogens. It can also be used for the analysis of cytotoxic effects of anticancer drugs, drug resistance, cytotoxic pharmaceutical compounds, and other toxic agents. |
Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat (10,000 cells/well) cells in 96 well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Fluoro IndoBluTM (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530nm and Em: 590nm.
PBMC proliferation in response to mitogen Concanavalin A (Con A) stimulation measured by Fluoro IndoBluTM. PBMC at 15,000 cells were cultured for 3 days in the presence of titrated amounts of Con-A and tested for proliferation using the Fluoro IndoBluTM assay. Fluoro IndoBluTM(1/10 volume) was added to the cultured cells and incubated @ 37°C for 2h. Fluorescence was detected at Ex: 530nm and Em: 590nm.
| Kit contents: |
| 1. |
1 Bottle of IndoBluTM reagent: 10mL. Upon arrival store at 2-8°C. |
| 2. |
Optional: 96-well clear-bottomed Black plate for fluorescent read-out. |
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