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Fluoro EPO™ 
Fluorescent Eosinophil Peroxidase Detection Kit
Key Benefits:
  • Can monitor multiple time points to follow kinetics.
  • One-step, no wash assay.
  • Adaptable for High Throughput format.
  • Highly Sensitive.
  • Applications - Fluorescent Plate Reader.
Assay Principle

Eosinophil peroxidase (EPO) is the most abundant enzyme found in eosinophils. It is the major cytotoxic agent released by activated eosinophils and uses hydrogen peroxide to generate reactive oxidants from halides and pseudo halide thiocyanate [1,2]. Eosinophils peroxidase has been shown to have antimycobacterial activity [3], however it is also implicated in tissue damage that occurs in asthma and other diseases [4,5]. Currently, the function of eosinophil involvement in the immune response is being redefined. Once considered a cell involved in host protection of parasitic infection, eosinophils multiple functions as leukocytes involved in the initiation and propagation of diverse inflammatory responses are being investigated. Eosinophils are further involved as modulators of innate and adaptive immunity [6].

H2O2 + Detection reagent (non-fluorescent)+ EPO ---------> fluorescent analog

Excitation:530-571nm; Emission: 590-600nm
EPO


Figure 1. Eosinophil peroxidase was titrated in 1X reaction buffer and 50uL of each point was added in triplicate to 96 black wells. 50uL of reaction cocktail was added and the reaction was incubated in the dark at room temperature for 30 minutes. 

Fluorescence was read at 530nm excitation and emission detected at 590nm.

Kit contents (for 100 assays)
1. 10X Assay Buffer: 60ml Part 3002
2. Detection reagent: One vial for 100 assays Part 4016
3. Hydrogen Peroxide: 1000µL of a stabilized 3% solution Part 3012
4. Eosinophil Peroxidase: 100µL in 1 vial at 10 units/ml Part 6016

Product Catalog # Size
Fluorescent Eosinophil Peroxidase (EPO) Detection Kit FLEPO 100-2 100