GENTAUR - Research solutions for the academic, biotechnology and pharmaceutical industries

Carboxyfluorescein Caspase Detection Kits™ 
Apoptosis detection and in situ labeling of active caspase in live cells.

• Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition.
• Cell permeablity permits direct visualization of cytosolic apoptotic events.
• Apoptotic cell population does not diminish over time.
• Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure.
• Works in diverse cell lines: human, rodent, Drosophila.
• Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells.
• Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments.
• Yields both quantitative and qualitative results. Gives strong signal with little background noise.
• Mark activity across the range of caspase proteins. Poly-caspase and caspase-specific assays to target caspases 1, 2, 3, 6, 8, 9, or 10 are available.
• Applications - Flow Cytometry, Fluorescence Plate Reader, Fluorescent Microscopy

APO LOGIX Carboxyfluoroscein Caspase Detection Kits label active caspases in living cells undergoing apoptosis. Gentaur’s probes utilize carboxyfluorescein(FAM)-labeled peptide fluoromethyl ketone (FMK) caspase inhibitors (FAM-peptide-FMK). These FAM-peptide-FMK compounds are both cell permeable and non-cytotoxic during the course of the assay and thus allow the detection of active caspases in living cell systems.