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Neomycin B sulfate, EvoPure®

Description

Neomycin is a broad spectrum aminoglycoside antibiotic. Neomycin is freely soluble in water (50mg/mL), and sparingly soluble in ethanol and methanol (0.10 mg/mL and 0.23 mg/mL respectively).

We offer five forms of neomycin: neomycin sulfate USP (N003), neomycin sulfate, EP (N012) neomycin B sulfate EvoPure® (N019), neomycin C sulfate, EvoPure® (N022), and neomycin with 10 mg/mL neomycin in 0.9% NaCl (N020).

Mechanism:
Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

Spectrum:
Neomycin is a broad spectrum antibiotic, however, it is mostly used against gram negative bacteria.

Microbiology Applications:
The broad spectrum antibiotic neomycin is used as selection agent in the genetic transformation process the same way as kanamycin. The resistance gene (NPT II) used in kanamycin mediated selection is exploited by neomycin mediated selection as well (Aragão, 2009).

Neomycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

Pseudomonas aeruginosa 0.5 µg/mL – 64 µg/mL
Haemophilus influenzae 1.6 µg/mL – 6.3 µg/mL
For a complete list of neomycin MIC values, click here.
In addition to its use in AST, neomycin sulfate is used for food testing in TSN agar to select for Clostridium perfringins and inhibit growth of Enterobacteria and Clostridium bifermentans.

Plant Biology Applications:
Neomycin is commonly used in negative selections for plants which have been successfully been transformed with a plasmid conferring resistance via Agrobacterium mediated transformation.

Mammalian Biology Applications:
Neomycin is routinely used to select for cells containing resistance plasmids such as pcDNA3 in common cell lines including AtT-20. For complete additional information on your cell culture needs, please visit our cell-culture database.

Form:
Powder

Purity Level:
≥99.0%

Price: (EUR)

Packaging: Regular Price: Promo Price:
1 x 10mg 322.22 113.10