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Kanamycin A sulfate, EvoPure®

Description

Kanamycin is an aminoglycoside antibiotic often used to select for plants (Agrobacterium mediated transformation) and bacteria which have been successfully transformed with a plasmid conferring kanamycin resistance. Kanamycin is very soluble in aqueous solution at 92.3 mg/mL.

Standard grade kanamycin is composed of a mixture of three different fractions: kanamycin A, B, and C. We offer five forms of kanamycin: kanamycin sulfate (K008), kanamycin acid sulphate (K004), kanamycin A sulfate, EvoPure® (K013), kanamycin B sulfate (K002), and kanamycin B sulfate, EvoPure® (K014). EvoPure® products are purified single antibiotic fractions, most >99% pure. High purity EvoPure® kanamycin products can be used to analyze the specific effects of individual kanamycin fractions.

Mechanism:
Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

Spectrum:
Kanamycin is a broad spectrum antibiotic, however, it is mostly used against aerobic gram negative bacteria.

Microbiology Applications:
Kanamycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

Salmonella enteritidis 62.5 µg/mL
Escherichia coli 31.2 µg/mL
For a complete list of kanamycin MIC values, click here.
Media Supplements
Kanamycin can be used as a selective agent in several types of isolation media:


Kanamycin Aesculin Azide Agar - Enterococci isolation in food

Plant Biology Applications:
Kanamycin is often used in Agrobacterium mediated transformation using the npt II gene as selection marker. Kaur and Bansal (2010) used kanamycin in combination with cefotaxime to control bacterial growth while transforming tomato.

Form:
Powder

Purity Level:
≥99.0%

Price: (EUR)

Packaging: Regular Price: Promo Price:
1 x 100 mg 322.22 113.10