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G418 disulfate, EvoPure®

Description

G418 disulfate also known as geneticin, is an aminoglycoside antibiotic commonly used in plant and mammalian cell culture as a selectable marker. Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

We offer three forms of G418: G418 disulfate (G001), G418 disulfate, EvoPure (G030), and G418 disulfate solution (G020). G418 disulfate and G418 disulfate, EvoPure® are soluble in aqueous solution. G418 disulfate solution is G418 dissolved in water at 50 mg/mL. EvoPure® products are purified single antibiotic fractions, most >99% pure.

Mechanism:
G418 blocks polypeptide synthesis in eukaryotic cells by binding irreversibly to 80S ribosomes and therefore disrupting their proofreading capability.

Plant Biology Applications:
Dong and McHughen (1992) compared the use of kanamycin and geneticin as a selection agent in the genetic transformation of flax by Agrobacterium mediated transformation. Experiments in sweet potato showed G418 to be a more reliable selection agent with a higher transformation efficiency compared to kanamycin.

Mammalian Biology Applications:
Resistance to G418 is expressed by the neo gene on transposon Tn5 which encodes amino-glycoside 3’-phosphotransferase. Tn5 carrying the neo gene can recombine with host DNA providing it with G418 resistance. G418 can be used gene selections for mammalian and yeast cells. For this application, a kill curve is typically performed to determine the minimum effective G418 concentration to kill non-resistant cells. For more information regarding G418 kill curves, click here. For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database.

Form:
Powder

Purity Level:
≥99.0%

Price: (EUR)

Packaging: Regular Price: Promo Price:
2 x 1 g 277.00 97
1 x 5 g 552.38 193.85
10 x 5 g 4603.20 1615.50