Protein expression

Protein expression systems for expression in e. coli, yeast and mammalian cells

 

Expression in E. coli

Expression vectors for protein expression in E. coli

 

 

QuickLyse pEAS1-a expression vector

Price (€): 560

Applications

  • Expression of recombinant proteins in E. coli
  • One-step cell lysis and release of protein into supernatant
  • Compatible with all purification tags

Benefits

  • Recombinant proteins are purified directly from the culture supernatant
  • No need for lysozyme treatment or sonication
  • Gentle extraction conditions ensure integrity and activity of the recombinant protein
  • Suitable for high-throughput protein expression

 

 

 

Expression vectors for Escherichia coli  

Applications

  • Tightly regulated expression of recombinant proteins in E. coli
  • Fermentation studies
  • Antibiotic-free cultivation using parDE operon


Benefits

  • Ultra-tight regulation and high levels of expression due to Pm/xylS regulon
  • Compatible with most expression vectors, including pET, pUC and pBAD series
  • Wide choice of antibiotic resistance markers
  • RK2 origin allows vector propagation in more than 60 gram negative species

Price (€):

Product name

Description

410

pVB1 expression vector

Promoter: Pm/xylS | Origin: RK2 | Resistance 1: Ampicillin | Resistance 2: -

410

pVB2 expression vector

Promoter: Pm/xylS | Origin: RK2 | Resistance 1: Ampicillin | Resistance 2: -

410

pVB3 expression vector

Promoter: Pm/xylS | Origin: RK2 | Resistance 1: Ampicillin | Resistance 2: -

410

pVB4 expression vector

Promoter: Pm/xylS | Origin: RK2 | Resistance 1: Tetracyclin | Resistance 2: -

410

pVB5 expression vector

Promoter: Pm/xylS | Origin: RK2 | Resistance 1: Kanamycin | Resistance 2: -

410

pVB6 expression vector

Promoter: - | Origin: RK2 | Resistance 1: Ampicillin | Resistance 2: Chloramphenicol

410

pVB7 expression vector

Promoter: - | Origin: RK2 | Resistance 1: Ampicillin | Resistance 2: Tetracyclin

410

pVB8 expression vector

Promoter: - | Origin: RK2 | Resistance 1: Ampicillin | Resistance 2: Kanamycin

410

pVB9 expression vector

Promoter: - | Origin: RK2 | Resistance 1: Ampicillin | Resistance 2: parDE

 

Expression in yeast

Expression system for protein expression in yeast

 

 

 

KickStart protein expression kit Price (€): 970

Applications

  • Expression of recombinant proteins in S. cerevisiae
  • Expression of membrane proteins and secreted proteins

Benefits

  • Protein expression in rich medium (YPD)
  • Self-inducing system, no addition of inducer required
  • Protease deficient strain for higher protein yields
  • One-step purification using the popular Strep tag

 

 

 

Yeast expression vectors  

Applications

  • Expression of recombinant proteins in S. cerevisiae
  • Expression of membrane proteins and secreted proteins
  • Inducible expression

Price (€):

Product name

Description

560

p427-TEF yeast expression vector

TEF promoter | High copy number plasmid | G418 selection

560

p417-CYC yeast expression vector

CYC1 promoter | Low copy number plasmid | G418 selection

560

pTEF-MF yeast expression vector

TEF1 promoter | High copy number plasmid | Secretion of protein

560

pGAL-MF yeast expression vector

GAL1-L promoter | High copy number plasmid | Secretion of protein

 

 

 

pFunk yeast expression vectors  
  • General purpose yeast expression vectors
  • Wide choice of promoters and auxotrophic markers
  • Constitutive and inducible expression
  • Rich multiple cloning site for easy subcloning of cDNA inserts

Price (€):

Product name

Description

560

p413CYC yeast expression vector

Promoter: CYC1 | Origin: CEN/ARS | Marker: HIS3

560

p413GAL-S yeast expression vector

Promoter: GAL1-S | Origin: CEN/ARS | Marker: HIS3

560

p413GAL-L yeast expression vector

Promoter: GAL1-L | Origin: CEN/ARS | Marker: HIS3

560

p414CYC yeast expression vector

Promoter: CYC1 | Origin: CEN/ARS | Marker: TRP1

560

p414TEF yeast expression vector

Promoter: TEF1 | Origin: CEN/ARS | Marker: TRP1

560

p414GAL-S yeast expression vector

Promoter: GAL1-S | Origin: CEN/ARS | Marker: TRP1

560

p414GAL-L yeast expression vector

Promoter: GAL1-L | Origin: CEN/ARS | Marker: TRP1

560

p415CYC yeast expression vector

Promoter: CYC1 | Origin: CEN/ARS | Marker: LEU2

560

p415ADH yeast expression vector

Promoter: ADH1 | Origin: CEN/ARS | Marker: LEU2

560

p415GAL-S yeast expression vector

Promoter: GAL1-S | Origin: CEN/ARS | Marker: LEU2

560

p415GAL-L yeast expression vector

Promoter: GAL1-L | Origin: CEN/ARS | Marker: LEU2

560

p416CYC yeast expression vector

Promoter: CYC1 | Origin: CEN/ARS | Marker: URA3

560

p416GAL-S yeast expression vector

Promoter: GAL1-S | Origin: CEN/ARS | Marker: URA3

560

p416GAL-L yeast expression vector

Promoter: GAL1-L | Origin: CEN/ARS | Marker: URA3

560

p423CYC yeast expression vector

Promoter: CYC1 | Origin: 2micron | Marker: HIS3

560

p423GAL-S yeast expression vector

Promoter: GAL1-S | Origin: 2micron | Marker: HIS3

560

p423GAL-L yeast expression vector

Promoter: GAL1-L | Origin: 2micron | Marker: HIS3

560

p424CYC yeast expression vector

Promoter: CYC1 | Origin: 2micron | Marker: TRP1

560

p424ADH yeast expression vector

Promoter: ADH1 | Origin: 2micron | Marker: TRP1

560

p424TEF yeast expression vector

Promoter: TEF1 | Origin: 2micron | Marker: TRP1

560

p425CYC yeast expression vector

Promoter: CYC1 | Origin: 2micron | Marker: LEU2

560

p425ADH yeast expression vector

Promoter: ADH1 | Origin: 2micron | Marker: LEU2

560

p425TEF yeast expression vector

Promoter: TEF1 | Origin: 2micron | Marker: LEU2

560

p425GAL-S yeast expression vector

Promoter: GAL1-S | Origin: 2micron | Marker: LEU2

560

p425GAL-L yeast expression vector

Promoter: GAL1-L | Origin: 2micron | Marker: LEU2

560

p426CYC yeast expression vector

Promoter: CYC1 | Origin: 2micron | Marker: URA3

560

p426TEF yeast expression vector

Promoter: TEF1 | Origin: 2micron | Marker: URA3

560

p426GAL-S yeast expression vector

Promoter: GAL1-S | Origin: 2micron | Marker: URA3

560

p426GAL-L yeast expression vector

Promoter: GAL1-L | Origin: 2micron | Marker: URA3

 

 

 

KickStart expression system vectors  

Applications

  • Expression of recombinant proteins in S. cerevisiae
  • Expression of membrane proteins and secreted proteins


Benefits

  • Protein expression in rich medium (YPD)
  • Self-inducing system, no addition of inducer required
  • Protease deficient strain for higher protein yields

Price (€):

Product name

Description

560

pKS1 tagless expression vector

Promoter: ADH2 | Origin: 2micron | Marker: KanMX (G418 selection) | Tag: -

560

pKS1-ST Strep-tag expression vector

Promoter: ADH2 | Origin: 2micron | Marker: KanMX (G418 selection) | Tag: Strep

560

pKS1-ST-Gal control vector

Promoter: ADH2 | Origin: 2micron | Marker: KanMX (G418 selection) | Tag: Strep-Gal (control)

560

pKS2-ST Strep-tag secreted expression vector

Promoter: ADH2 | Origin: 2micron | Marker: KanMX (G418 selection) | Tag: Strep (secreted protein)

 

 

 

Yeast expression strains  

Applications

  • Expression of recombinant proteins in yeast
  • Intracellular and secreted expression
  • Protease deficient strains

Price (€):

Product name

Description

390

DSY-5 yeast expression strain

Protease deficient yeast strain (Pep4/ Prb1)

390

DSY-6 yeast expression strain

Protease deficient yeast strain (Pep4/ Prb1/Prc1)

 

 

Expression in mammalian cells

 

Expression system for protein expression in mammalian cells

  • Expression of recombinant proteins in mammalian cells
  • Transient and stable expression
  • Follow-up co-immunoprecipitation studies after DUALhybrid (yeast two-hybrid), DUALmembrane and DUALhunter screens

 

 

 

Mammalian expression vectors  

Applications

  • Expression of recombinant proteins in mammalian cells
  • Transient and stable expression
  • Follow-up co-immunoprecipitation studies after DUALhybrid (yeast two-hybrid), DUALmembrane and DUALhunter screens

Price (€):

Product name

Description

560

pHA-MEX mammalian expression vector

CMV Promoter | HA tag (N-terminal) | transient and stable expression vector

560

pMEX-HA mammalian expression vector

CMV Promoter | HA tag (C-terminal) | transient and stable expression vector

 

 

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