Column
Optimisil HPLC Column catalog
C18 Reverse phase HPLC
Characteristics
- Highly controlled chemistry of monolayer formation and end-capping
- Extremely high column-to-column reproducibility
- High selectivity and efficiency
MF-C18 |
| MF-C18 uses full coverage bonded silica packing, which provides exceptionally high stability. The unique mono-functional bonding chemistry of MF-C18 avoids the formation of multiple C18 layers. Such uniform stationary phase allows the separation to achieve high selectivity and high efficiency. |
AQ-C18 |
| AQ-C18 uses full coverage bonded silica packing , which provides exceptionally high stability. Compatible with 100% aqueous mobile phase suitable for separations of acidic, neutral and basic organic compounds, as well as pharmaceuticals, peptides, and others. |
HL-C18 |
| HL-C18 uses a proprietary ODS bonding chemistry to a high surface area silica gel to achieve long retention time and high loading capability. The ODS density is specially designed to achieve optimal selectivity for both hydrophobic and hydrophilic compounds. The proprietary end-capping chemistry maximizes consumption of silanol groups on the silica surface,and minimizes the free silanol groups to an undetectable leve. Such unique bonding chemistry allows the phase to achieve high peak symmetry even for basic compounds. HL-C18 phase is compatible with 100% aqueous mobile phases. |
LP-C18 |
| The uniform stationary phase allows the separation to achieve high selectivity and high efficiency. Pore size selection of 200 and 300Å and high compatibility with 100% aqueous phases make LP-C18 columns ideal for high resolution mapping of peptides and separation of natural and synthetic peptides and small proteins. The specially designed surface bonding chemistry and the pore sizes allow for extended retention and selectivity for polar and hydrophilic compound, such as peptides and amino acids. |
MC-C18 |
| Utilizing highest purity and enhanced mechanical stability silica and pure bonding reagents, MC-C18 bonded phases have been innovatively and specially designed to ensure maximum surface coverage and full end-capping , which leads to carbon content as high as 19.0%. The bonding chemistry is completely controlled that results in very reliable column-to-column reproducibility. The maximum surtace coverage allows MC-C18 to have exceptional stability, resulting in high ph stability in the range of1.5 to 10.5. |
Other Reversed Phase HPLC
Characteristics
- Highly controlled chemistry of monolayer formation and end-capping
- Extremely high column-to-column reproducibility
- High selectivity and efficiency for separations
- Full coverage bonded silica packing to achieve the exceptionally high stability
MF-C8 | MF-C8 phase is synthesized with monomeric and fully endcapped chemistry. The uniform octyl stationary phase allows high efficiency with lower hydrophobicity compared to ODS phase MF-C8 phase is suitable for separating compounds with a wide range of hydrophobicity. It is highly recommended for separating the compounds which are too strongly retained on C18 phases. | |
LP-C8 | LP-C8 phase is made of monomeric and fully endcapped chemistry. The uniform stationary phase allows the separation to achieve high selectivity and high efficiency. LP-C8 packings of 300 A pore size are ideal for high resolution Mapping of peptides and separation of natural and synthetic peptides and small proteins. | |
MF-C4 | onomeric and fully endcapped MF-packing is bonded with butyl group that leads to moderate hydrophobicity. MF-C4 columns have the great selectivity and peak symmetry with moderate retention for separations of acidic, neutral and basic organic compounds, such as pharmaceuticals, peptides and organic acids. | |
LP-C4 | Monomeric and fully endcapped MF-C4 packing is bonded with butyl group that leads to moderate hydrophobicity. LP-C4 packings of 300A pore size are ideal for high resolution mapping of peptides and separation of natural and synthetic peptides and small proteins. | |
MF-Phenyl | MF-Phenyl packing materials are bonded with propyl phenyl groups that enable special interaction with ring structured compounds. The monomeric bonding chemistry gives very high efficiency and high resolution separations. MF-Phenyl phase is suitable for separations of acidic, neutral and basic organic compounds, as well as the pharmaceuticals. |
Normal Phase LC
Characteristics
- Highly controlled chemistry of monolayer formation and end-capping
- Extremely high column-to-column reproducibility
- High selectivity and efficiency for separations
Preparation and Semi preparation column
CVC | pore size Å | Surface area ㎡/gm | ligand type | phase | available pore size | silica type | end cap | carbon load |
small molecules | 100 | 190 | monofunctional | C8, C18, Silica | 5 um | A | fully endcapped | C8:7 % C18:12 % |
Acid/Basic compounds | 100 | 430 | monofunctional | C18 | 3,4.5,10 | B(ultra pure) | proprietary and extremely exhaustive(fully capping) | 18% |
polar compound | 120 | 120 | monofunctional | C8,C18,Silica,Cyano,Phenyl | 4.5,10 | B | Extremely well | 18% |
Peptide (19 KDa/less) | 200 | 200 | monofunctional | C4,18 | 4.5,10 | B | fully endcapped | C4:4.5 % C18: 14 % |
Protein | 300 | 100 | monofunctional | C4,C18 | 4.5, 10 | B | fully endcapped | C4:3 % C18: 8 % |
hydrophilic | 120 | 330 | monofunctional | C8,C18 | 3,4,5,10 | B | unique polar and bulky end capping | 18% |
UHPLC column
UHPLC silica packings use high purity (<10 ppm metals), spherical silica with the particle selection of 1.8 and 2.2 mm and the pore size of 120 Å. Their bonded
phases include C18, C8, C4, Phenyl, Amino, Cyano, strong cation, and HILIC. Their unique monofunctional bonding chemistry allows high efficiency, high
selectivity and high speed separation. All sub-2 micron particles and bonded phases have excellent resistance to high pressure (>10,000 psi). As an example in Figure 1, 2.2 μm MF-C18 shows a reduced plate height of 2.22 μm, which is equivalent to plate number of 200,448 per meter.
Characteristics
- Most comprehensive selection of the stationary phases
- Highly controlled chemistry of monolayer formation and end-capping
- High column-to-column reproducibility
- High mechanical stability to resist the pressure as high as up to 10,000 psi
- High resolution, efficiency and selectivity for separations
- Suitable for separations of acidic, neutral and basic compounds, peptides, and proteins
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