SIGNAL ENHANCING WASH BUFFERS

A Room temperature retrieval
and stain amplification without pre-treatments

ADVANCED FEATURES
• Simple to use, employ as the rinse buffer in place of PBS for rinsing in between incubation steps
• Amplify staining signal by 2-4 folds with no additional steps added to the immunostaining procedure
• Cost effective: Allows 2-4 folds increase for primary antibody dilution
• Time Saver : Allows for decrease in primary antibody incubation time
• Allows for decrease in chromogen/substrate incubation time
• Fixation independent; Amplifies staining regardless of the type of fixative employed
• Eliminates enzyme pre-digestion
• Minimizes the need for high heat antigen recovery when used with Gentaur Staining Systems
• Minimizes false negative staining
• Automation compatible
• Applicable to ELISA and flow cytometric procedures

USER FRIENDLY PROTOCOL
Gentaur Enhancing Wash Buffers only require 5 seconds rinse in between incubation steps when used with Gentaur STAT-Q and HISTO-STAT ( 2-step) staining systems. Gentaur Enhancing Wash Buffers may be employed with other systems, observe the manufacturer’s instruction for the required time and number of washes recommended for the system.

1. Rinse specimens with Gentaur Enhancing Wash Buffer prior to applying primary antibody;
2. Incubate with primary antibody per manufacturer’s instructions;
3. Rinse with Gentaur Enhancing Wash Buffer;
4. Incubate with secondary antibody;
5. Rinse with Gentaur Enhancing Wash Buffer;
6. Incubate with Enzyme label;
7. Rinse with Gentaur Enhancing Wash Buffer;
8. Incubate with substrate/chromogen of choice;
9. Rinse with water;
10. Counterstain and mount.

For ELISA assays
Gentaur Enhancing Wash Buffers can be used for washing ELISA plates, however, some non-ionic detergent should be added to the solution.

SIGNAL ENHANCER WASH BUFFERS ARE AVAILABLE IN:

Enhancing Wash Buffer for retrieving
AEC and DAB stains