Gentaur's Products: Simple, Fast, and Background-free IHC staining of Human and Animal Tissues
For IHC staining in 60 minutes
| PAN CYTOKERATIN HUMAN BREAST CARCINOMA |
bcl-2 | CYTOKERATIN 7 HUMAN BREAST CARCINOMA |
CD 45 HUMAN TONSIL |
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For Wash- Free, Background-Free STAT (rapid) immunostaining in 2 easy steps and under one hour. Free of avidin, biotin, and streptavidin
A single kit system for animal and human tissue staining
ABOUT POLYMER HISTO-STAT Kits
HISTO-STAT (2-step staining system) is a Non-biotin & Non-streptavidin system developed for wash-free, background-free IHC stainingin 60 minutes. Does NOT require Protein/ serum Blocking.
Gentaur Polymer HIsto-Stat kits only involve 2 staining steps post incubation with the primary antibody; an incubation with the Polymer reagent and, a short incubation step (5 minutes) with thesubstrate/chromogen of choice.
Histo-stat uses one less reagent, one less incubation step and one less rinse step.
UNIQUE FEATURES
- No Background. No serum or protein blocking step required
- No re-titration of current primary antibodies required, simply use HISTO-STAT Polymer kits with your current primary antibody titers
- Total staining time of only 60 minutes for paraffin sections and 20-30 minutes for frozen sections
- Wash-Free, only 5 second rinses in between each incubation step.
- Free of biotin; No avidin/ biotin blocking required
- Short incubation steps
- 3 years of shelf life
- Highly amplified, equates the sensitivity of biotin-streptavidin system
HISTO-STAT USER FRIENDLY PROTOCOL (total staining time: 60 minutes for paraffin section, 20 minutes for frozen sections)
No protein blocking step or extensive rinses are required when staining with Gentaur staining systems.
Post Deparaffinization:
- Incubate the section or smear for 20 minutes with primary antibody, rinse once with PBS or Gentaur “Signal Enhancing Wash Buffer” for 5 seconds;
- Incubate with the Peroxidase (HRP) or alkaline phosphatase Second Step Reagent for 20 minutes, rinse once with PBS or Gentaur “Signal Enhancing Wash Buffer” for 5 seconds;
- Incubate with Gentaur substrate/chromogen of choice (AEC or DAB for HRP enzyme label) and Gentaur Brown, Fast Red, or other substrate/chromogen available for alkaline phosphatase enzyme for 5-10 minutes, rinse with water;
- Counterstain and Mount.
For Immunostaining in 60 minutes
POLYMER HISTO-STAT staining systems are available in Complete Staining Systems and in Individual Components
HISTO-STAT, No-wash, No-background Complete Staining Systems include all staining components required including substrate/ chromogen of choice.
HISTO-STAT Complete Staining Systems are available in:
HISTO-STAT Peroxidase Multivalent Complete System (Large Volume) HISTO-STAT Peroxidase Multivalent Complete System (Small Volume)
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HISTO- STAT Alkaline phosphatase Multivalent Complete systems HISTO- STAT Alkaline phosphatase Multivalent Complete systems
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COMPONENTS
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| Item# | Product Description | Unit |
| NB312L | HISTO-STAT Multivalent Second Step Reagent (HRP) for staining both MOUSE & RABBIT primary antibodies | 50 ml |
| NB312L-15 | HISTO-STAT Multivalent Second Step Reagent (HRP) for staining both MOUSE & RABBIT primary antibodies | 20 ml |
| NB317L-15 | HISTO-STAT Multivalent Second Step Reagent (Alkaline phosphatase) for staining both MOUSE & RABBIT primary antibodies | 20 ml |
| NB312C-15 | HISTO-STAT Monovalent-Chicken Second Step Reagent (HRP) for staining CHICKEN primary antibodies | 15 ml |
| NB312M-15 | HISTO-STAT Monovalent-Mouse Second Step Reagent (HRP) for staining MOUSE primary antibodies | 15 ml |
| NB312R-15 | HISTO-STAT Monovalent-Rabbit Second Step Reagent (HRP) for staining RABBIT primary antibodies | 15 ml |
| NB312S-15 | HISTO-STAT Monovalent-Sheep Second Step Reagent (HRP) for staining SHEEP primary antibodies | 15 ml |
| NB312G-15 | HISTO-STAT Monovalent-Goat Second Step Reagent (HRP) for staining GOAT primary antibodiess | 15 ml |
| NB312H-15 | HISTO-STAT Monovalent-Hamster Second Step Reagent (HRP) for staining HAMSTER primary antibodies | 15 ml |
| NB312T-15 | HISTO-STAT Monovalent-Rat Second Step Reagent (HRP) for staining Rat primary antibodies | 15 ml |
| NB312MRA-15 | HISTO-STAT Monovalent-Rat Second Step Reagent (HRP) MOUSE ADSORBED for staining RAT primary antibodies in Mouse tissue | 15 ml |
| NB312RMA-15 | HISTO-STAT Monovalent-Mouse Second Step Reagent (HRP) RAT ADSORBED for staining MOUSE primary antibodies in Rat tissue | 15 ml |
| NB317L | HISTO-STAT Multivalent Second Step Reagent (Alkaline phosphatase) for staining both MOUSE & RABBIT primary antibodies | 50 ml |
