The Total RNA Extraction Mini Kit (Blood/Cultured Cells) is designed specifically for purifying total RNA from fresh whole human blood and cultured cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase (optional DNase treatments can be followed to remove unwanted DNA residue). RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column and once any contaminants have been removed, using the Wash Buffer, the purified total RNA is eluted by RNase-free Water. The entire procedure can be completed within 30 minutes.
- Sample :
up to 300 µl of whole blood
cultured cells (up to 5 x 106)
bacterial cells (up to 1 x 109)
fungal cells (up to 5 x 107) - Format :
spin column (centrifuge) - Yield :
5-30 μg Operation time: within 30 minutes
RT-PCR, Northern blotting, primer extension, mRNA selection cDNA synthesis
The quality of the Total RNA Extraction Mini Kit (Blood/Cultured Cells) is tested on a lot-to-lot basis by isolating total RNA from blood and cultured cell samples. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.
| RBC Lysis Buffer | 30 ml |
| RB Buffer | 30 ml |
| RT Buffer | 12.5 ml |
| W1 Buffer | 6 ml |
| Wash Buffer* | 50 pcs |
| RNase-Free water | 50 pcs |
| RB Column | 100 pcs |
| 2 ml Collection Tube | 50 pcs |
*Add 50 ml of absolute ethanol to the AD Buffer prior to initial use |
|
| RBC Lysis Buffer | 30 ml |
| RB Buffer | 30 ml |
| RT Buffer | 12.5 ml |
| W1 Buffer | 6 ml |
| Wash Buffer* | 50 pcs |
| RNase-Free water | 50 pcs |
| RB Column | 100 pcs |
| 2 ml Collection Tube | 50 pcs |
*Add 50 ml of absolute ethanol to the AD Buffer prior to initial use |
|
| RBC Lysis Buffer | 30 ml |
| RB Buffer | 30 ml |
| RT Buffer | 12.5 ml |
| W1 Buffer | 6 ml |
| Wash Buffer* | 50 pcs |
| RNase-Free water | 50 pcs |
| RB Column | 100 pcs |
| 2 ml Collection Tube | 50 pcs |
*Add 50 ml of absolute ethanol to the AD Buffer prior to initial use |
|
