96-Well Gel/PCR DNA Fragment Extraction Kit
Description

The 96-Well Gel/PCR DNA Fragment Extraction Kit provides a high-throughput method to recover or concentrate DNA fragments from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fibre matrix of each well of the plate. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer or TE. Salts, enzymes and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. The entire procedure can be completed in 30-40 minutes.

Features

  • Sample :  
    up to 50 mg of agarose gel slice,
    up to 50 μl of PCR products

  • Format :
    96-well plate (centrifuge/vacuum manifold)

  • DNA Size :
    100 bp - 10 Kb

  • Recovery :
    up to 85% for gel extraction within 40 minutes,up to 90% for PCR cleanup within 30 minutes
Applications

PCR, fluorescent/radioactive sequencing, restriction enzyme digestion, DNA labeling and ligation

Quality Control

The quality of the 96-Well Gel/PCR DNA Fragment Extraction Kit is tested on a lot-to-lot basis by isolating DNA fragments of various sizes from either aqueous solutions or agarose gels. The purified DNA is checked by electrophoresis

Results

As reflected by the Colony Number (45, 31, 40 and 35), transformation and ligation are successful for both Gentaur Biotech and Competitor Q. Gentaur Biotech restriction enzyme digestion efficiency is equal to that of the competitor as no colony could be observed in the Ampicillin plate in the absence of T4 DNA ligase for either kit.

Specification
Store at

 

# FYG205-004 (4 plates)
Binding Buffer 120 ml
W1 Buffer 130 ml
Wash Buffer 25 ml
Elution Buffer 30 ml
DNA Binding Plate 4 pcs
0.35 ml Collection Plate 4 pcs
Adhesive Film 8 pcs
# FYG205-010 (10 plates)
Binding Buffer 320 ml
W1 Buffer 130 ml × 2
Wash Buffer 50 ml
Elution Buffer 60 ml
DNA Binding Plate 10 pcs
0.35 ml Collection Plate 10 pcs
Adhesive Film 20 pcs
GENTAUR Europe BVBA, Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 | Fax 0032 16 50 90 45
Connect With Us:
Copyright © Gentaur - Molecular products 2014