The 96-Well Gel/PCR DNA Fragment Extraction Kit provides a high-throughput method to recover or concentrate DNA fragments from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fibre matrix of each well of the plate. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer or TE. Salts, enzymes and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. The entire procedure can be completed in 30-40 minutes.
PCR, fluorescent/radioactive sequencing, restriction enzyme digestion, DNA labeling and ligation
The quality of the 96-Well Gel/PCR DNA Fragment Extraction Kit is tested on a lot-to-lot basis by isolating DNA fragments of various sizes from either aqueous solutions or agarose gels. The purified DNA is checked by electrophoresis
As reflected by the Colony Number (45, 31, 40 and 35), transformation and ligation are successful for both Gentaur Biotech and Competitor Q. Gentaur Biotech restriction enzyme digestion efficiency is equal to that of the competitor as no colony could be observed in the Ampicillin plate in the absence of T4 DNA ligase for either kit.
# FYG205-004 (4 plates)
Binding Buffer | 120 ml |
W1 Buffer | 130 ml |
Wash Buffer | 25 ml |
Elution Buffer | 30 ml |
DNA Binding Plate | 4 pcs |
0.35 ml Collection Plate | 4 pcs |
Adhesive Film | 8 pcs |
Binding Buffer | 320 ml |
W1 Buffer | 130 ml × 2 |
Wash Buffer | 50 ml |
Elution Buffer | 60 ml |
DNA Binding Plate | 10 pcs |
0.35 ml Collection Plate | 10 pcs |
Adhesive Film | 20 pcs |