Description

Easy3-Plant RNA Isolation Kit provides a quick and easy 3 step method to isolate total RNA from plant samples. The unique RPR Buffer system ensures total RNA with high yield and good quality from most common plant samples and also samples high in polysaccharides. If a larger sample is processed, the buffer volume can be scaled proportionately, making this reagent not only very user friendly but also highly versatile. DNA phenol extraction is not required and the entire procedure can be completed in 2 hours.

Features

  • Sample: 100 mg (fresh plant tissues)
  • Format: reagent system
  • Yield: up to 80 μg
Applications

  • RT-PCR,
  • Northern blotting
  • cDNA synthesis and mapping
Quality Control

The quality of the Easy3-Plant RNA Isolation Kit is tested on a lot-to-lot basis by isolating total RNA from 100 mg of tissue samples. A minimum of 20 μg of total RNA is quantified with a spectrophotometer and checked by electrophoresis.

Additional Requirements

Microcentrifuge tubes (RNase-free), absolute ethanol for preparing 70% ethanol in water (RNase-free), chloroform, Isopropanol, mortar and pestle, RNase-free water, s-mercaptoethanol.

Specification
# FYG502-100P (100 preps)
RPR1 Buffer* 100 ml
RPR2 Buffer* 15 ml
# FYG502-500P (500 preps)
RPR1 Buffer* 500 ml
RPR2 Buffer* 75 ml

* If the buffers contain sediment, incubate at 65ºC for 10 minutes to dissolve.
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