Gentaur Sharp Protein Marker II (# FLP005-16P and # FLP005-96P) are the lyophilized form of our popular protein markers, # FYP005-250UL / # FYP005-500UL. Unlike traditional mixes that must be aliquoted, # FLP005-16P and # FLP005-96P are pre-aliquoted and lyophilized in ready-to-use form in a foil-covered, 48-well plate. Before use, users can simply puncture the foil cover with a pipette tip and add 5 µL water to one plate well. The marker mixes dissolve in few seconds and are immediately ready for loading onto SDS-PAGE gel. The marker mix is pre-denatured and stabilized in loading buffer, so no additional treatment is necessary before loading the reconstituted aliquot.

• Easy to use:
  just add water and load


• Convenient to store:
  stable at room temperature for one year


• No contamination risk:
  eliminates chances for contamination due to multiple withdrawals from the same vial


• Broad compatibility and transferable:
  compatible with standard SDS-PAGE methods and transfer well to both nitrocellulose and PVDF membranes

• Monitoring protein migration during SDS-PAGE
• Monitoring protein transfer onto membranes during Western blotting
• Approximate sizing of proteins on SDS-PAGE gels
• Acrylamide gels and Western blots

• The ladder contains two green reference bands at ~50 kDa and 10 kDa and one orange reference band of 70 kDa.
• Avoid multiple freeze/thaw cycles.
• Lot-to-lot variation of the apparent molecular weight of prestained proteins is < 10% depending on the molecular weight.
• Western blotting of large (>100 kDa) proteins may need longer transfer times or higher transfer voltages for efficient transfer.

After storage at room temperature and 37 °C for 3 months, lyophilized Sharp Protein Markers were resuspended in specified volumes of water and 5 μl of markers was run on SDS-PAG to ensure expected numbers of bands in SDS-PAGE (Tris-glycine buffer) without sign of protein degradation as compared to protein markers stored at -20 °C.

• Apply 5 μl/well on mini gels and 10 ~ 15 μl/well on large gels
• For blots, apply 1 ~ 3 μl/well on mini gels and 2 ~ 6 μl/well on large gels