RealStart DNA Polymerase Premix
Description

RealStart DNA Polymerase premix is an ultra-sensitive and convenient PCR premix product. It contains 2× concentrated solution of HotStart DNA polymerase, dNTPs, optimized buffers, and loading dye (optional) needed for PCR. The only step it takes to perform PCR with RealStart DNA Polymerase premix is to add DNA template and primers into the reaction mix. Since special HotStart DNA polymerase in the premix is activated after heating, it greatly reduces non-specific amplification when working with the premix at room temperature.
Features

  • Simple & time-saving
    Just add template of interest and primers into the RealStart DNA Polymerase Premix.

  • Less Contamination
    Reduce non-specific amplification caused by mispriming events that occur during setup and initial temperature increase.

  • High Sensitivity
    tested in amplification of a single gene copy.

  • Convenient 
    an excellent tool when working with high quantities of samples.

  • Sample Size
    work excellent for short DNA templates (size shorter than 600 bp).
Applications

  • High throughput hot-start PCR.
  • RT-PCR.
  • Highly specific amplification of complex genomic and cDNA templates.
  • Amplification of low copy DNA targets.
  • Generation of PCR products for TA cloning.
Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.

Quality Control

  • Nuclease activity is not detected after incubation of 1 μg λ /Hind III DNA with 5 units of RealStart DNA polymerase in 50 μl reaction buffer for 18 hours at 37 °C.
  • The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate tests. Functional test is performed by PCR.
Results

Selection Guide of Gentaur DNA Polymerases

 

Specification
Store at

 

# FYT101-100P (100 preps)
2× RealStart DNA Polymerase 1.25 ml
Premix (w/o loading dye) 0.1 U/μl
# FYT102-100P (100 preps)
2× RealStart DNA Polymerase 1.25 ml
Premix (w/ loading dye) 0.1 U/μl
Premix contains:
■ Hotstart Taq DNA polymerase
■ dNTPs mix (including dATP,
    dCTP, dGTP, dTTP)
■ 7.5 mM MgCl2
■ Loading dye (including
    bromophenol blue)

 

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