Streptavidin MagBeads
Description

The binding of streptavidin to biotin is one of the fastest and strongest known non-covalent biological interactions. Hence, mild denaturing conditions are generally required for the efficient elution of biotinylated biomolecules. Alternatively, biotinylated biomolecules bound to Streptavidin MagBeads can be used to intercept interacting target molecules such as proteins and nucleic acids. Impurities are easily removed by washing, and the enriched targets are eluted using less harsh elution conditions.

Features

  • Beads diameter: 0.771 μm
  • Absolute consistency
  • All you need is a tube and a magnet
  • All protocols can be easily automated
  • Extremely stable in ambient temperature
  • No centrifugation, precipitation, or filtration
  • Fewer handling steps and no sample dilution
  • Robust but gentle, preserving protein nativity
  • Optimal binding kinetics and minimal sample loss
  • Stable in detergents and biological buffers at ambient environment
  • Even distribution of biotinylated compound on surface for optimal binding results
  • Enable quantitative isolations
Applications

  • Proteins and nucleic acid separation
  • Parallel screening of proteins with high repeatability
  • Small or large-scale purification of proteins from cell lysate and various biological fluids
Quality Control

Each lot of Streptavidin MagBeads was tested for its association rate with nucleic acids. It also has to pass the concentration test by spectrophotometer before shipping.

Additional Materials Required

Protocol

Results

Qualitative analysis of HBV dsDNA using Streptavidin MagBeads system

Constructed HBV plasmids were serially diluted and bound by Streptavidin magnetic beads and analyzed with chemiluminescence. (Noted, 256× means 0.3125 ng DNA.)

Specification
Store at

 

Isolation
# FYG107-40P
(40 preps)

Streptavidin MagBeads (10 mg/ml) 1 ml
Binding & Washing Buffer 25.2 ml
Nucleic Acid Elution Buffer 2.1 ml
# FYG107-200P (200 preps)
Streptavidin MagBeads (10 mg/ml) 5 ml
Binding & Washing Buffer 126 ml
Nucleic Acid Elution Buffer 10.5 ml

 

Detection
# FYG108-200P
(200 preps)

Streptavidin MagBeads (10 mg/ml) 1 ml
Binding & Washing Buffer 70 ml
Bioactive Catalyst Solution 21 ml
Substrate A 2.1 ml
Substrate B 2.1 ml
# FYG108-1000P (1000 preps)
Streptavidin MagBeads (10 mg/ml) 5 ml
Binding & Washing Buffer 350 ml
Bioactive Catalyst Solution 105 ml
Substrate A 10.5 ml
Substrate B 10.5 ml

 

Isolation & Detection
# FYG109-120P
(Isolation: 20 preps; Detection: 100 preps)

Streptavidin MagBeads (10 mg/ml) 1 ml
Binding & Washing Buffer 47.6 ml
Nucleic Acid Elution Buffer 1.05 ml
Bioactive Catalyst Solution 10.5 ml
Substrate A 1.05 ml
Substrate B 1.05 ml

 

Buffer
# FYG110-ISO

Binding & Washing Buffer 126 ml
Nucleic Acid Elution Buffer 10.5 ml
# FYG110-DEC
Binding & Washing Buffer 350 ml
Bioactive Catalyst Solution 105 ml
Substrate A 10.5 ml
Substrate B 10.5 ml
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