The binding of streptavidin to biotin is one of the fastest and strongest known non-covalent biological interactions. Hence, mild denaturing conditions are generally required for the efficient elution of biotinylated biomolecules. Alternatively, biotinylated biomolecules bound to Streptavidin MagBeads can be used to intercept interacting target molecules such as proteins and nucleic acids. Impurities are easily removed by washing, and the enriched targets are eluted using less harsh elution conditions.
Each lot of Streptavidin MagBeads was tested for its association rate with nucleic acids. It also has to pass the concentration test by spectrophotometer before shipping.
Qualitative analysis of HBV dsDNA using Streptavidin MagBeads system
Constructed HBV plasmids were serially diluted and bound by Streptavidin magnetic beads and analyzed with chemiluminescence. (Noted, 256× means 0.3125 ng DNA.)
Isolation
Streptavidin MagBeads (10 mg/ml) | 1 ml |
Binding & Washing Buffer | 25.2 ml |
Nucleic Acid Elution Buffer | 2.1 ml |
Streptavidin MagBeads (10 mg/ml) | 5 ml |
Binding & Washing Buffer | 126 ml |
Nucleic Acid Elution Buffer | 10.5 ml |
Streptavidin MagBeads (10 mg/ml) | 1 ml |
Binding & Washing Buffer | 70 ml |
Bioactive Catalyst Solution | 21 ml |
Substrate A | 2.1 ml |
Substrate B | 2.1 ml |
Streptavidin MagBeads (10 mg/ml) | 5 ml |
Binding & Washing Buffer | 350 ml |
Bioactive Catalyst Solution | 105 ml |
Substrate A | 10.5 ml |
Substrate B | 10.5 ml |
Streptavidin MagBeads (10 mg/ml) | 1 ml |
Binding & Washing Buffer | 47.6 ml |
Nucleic Acid Elution Buffer | 1.05 ml |
Bioactive Catalyst Solution | 10.5 ml |
Substrate A | 1.05 ml |
Substrate B | 1.05 ml |
Binding & Washing Buffer | 126 ml |
Nucleic Acid Elution Buffer | 10.5 ml |
Binding & Washing Buffer | 350 ml |
Bioactive Catalyst Solution | 105 ml |
Substrate A | 10.5 ml |
Substrate B | 10.5 ml |