Deoxy+ Real-time SGentaurR Green RT-PCR system provides user with a rapid and simple way to quantify the expression of gene of interest based on Real-time PCR system containing SGentaurR Green. Gentaur’s Deoxy+ HiSpec RT, Hotstart DNA polymerase and all the components for Real-time SGentaurR Green RT-PCR are skillfully mixed within a single tube. Unique buffer system allows highly specific quantification by preventing the formation of nonspecific products and primer–dimers.
The performance of Deoxy+ Real-time SGentaurR Green RT-PCR Kit is tested in an RT reaction using human embryonic kidney cell lysate with primer d(T)18. The sensitivity of the kit is verified by the detection of B2M transcript after 35 cycles. The length of cDNA achieved is verified by detection of a 248 bp by DNA agarose gel and DNA sequencing.
# FYT504-50P (50 preps)
2× Deoxy+ Real-time SGentaurR Green RT PCR Premix, ROX | 0.625 ml |
Sterilized ddH2O | 1 ml |
2× Deoxy+ Real-time SGentaurR Green RT PCR Premix, ROX | 0.625 ml×2 |
Sterilized ddH2O | 1 ml×2 |