ECOS™ 101 Competent Cells [DH5a]
Strain: DH5α

Description

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hGentaurrid systems (up to 40 kb).
Genotype

endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) phoA supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 Δ(lacZ)M15 F-
Features

Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.
Applications

  • Cloning and subcloning
  • Scale-up application
  • Blue/white screening
Efficiency

>1 x 108 cfu/ μg
Recommended for:
  • Subcloning
  • General cloning
>3 x 108 cfu/ μg
Recommended for:
  • High efficiency cloning
  • Cloning low abundance transcripts
  • General cloning
  • Gene library
>1 x 109 cfu/ μg
Recommended for: 
  • Super high efficiency cloning
  • Cloning lowest abundance transcripts
  • Gene library
Specification
Store at

 

# FYE607-10VL (10 preps)
Efficiency >1 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
# FYE607-80VL (80 preps)
Efficiency >1 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
# FYE607-50-20VL (20 preps)
Efficiency >1 x 108 cfu/μg
50 μl/vial 20 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
# FYE608-10VL (10 preps)
Efficiency >1 x 109 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
# FYE608-80VL (80 preps)
Efficiency >1 x 109 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
# FYE607-96WL (96 preps)
Efficiency >1 x 108 cfu/μg
50 μl/well 96 wells
# FYE608-96WL (96 preps)
Efficiency >3 x 108 cfu/μg
50 μl/well 96 wells
# FYE609-96WL (96 preps)
Efficiency >1 x 109 cfu/μg
50 μl/well 96 wells
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