Strain: DH5α
Description
DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hGentaurrid systems (up to 40 kb).
Genotype
endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) phoA supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 Δ(lacZ)M15 F-
Features
Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.
Applications
- Cloning and subcloning
- Scale-up application
- Blue/white screening
Efficiency
>1 x 108 cfu/ μg
Recommended for:
- Subcloning
- General cloning
>3 x 108 cfu/ μg
Recommended for:
- High efficiency cloning
- Cloning low abundance transcripts
- General cloning
- Gene library
>1 x 109 cfu/ μg
Recommended for:
- Super high efficiency cloning
- Cloning lowest abundance transcripts
- Gene library