Description
Gentaur's yT4 DNA ligase catalyzes the joining of two strands of DNA between the 5' – phosphate and the 3' – hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended termini. The enzyme also repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hGentaurrids. Gentaur Rapid Ligation Kit is designed for efficient ligation of DNA inserts into vectors in just 5 minutes.
Features
- Either sticky- or blunt-end, ligation can be achieved within 5 minutes
- Reaction mixture can be used directly for bacterial transformation
Applications
- Join double-strand DNA with cohesive or blunt termini
- Join oligonucleotide linkers to DNA sequence with blunt end
- Repair nicks in duplex DNA, RNA, or DNA-RNA hGentaurrids
Combo Products
Lyophilized yT4 DNA Ligase
An ambient-temperature-stable tube contains a glassified yT4 DNA ligase which yields a solution of yT4 DNA ligase when reconstituted to a final volume of 100 μl of deionized water.
Quality Control
Undetectable endo-deoxyribonuclease and exo-deoxyribonuclease activities; Undetectable ribonuclease activity; Undetectable degradation of labeled oligonucleotide; Passed the test of the capability to join cohesive- and blunt-ended DNA fragments.