DNA Pure Kit
Description

The DNA Pure Kit is designed to purify or concentrate DNA products which have been previously isolated using a variety of DNA isolation methods. The unique DNA Pure Buffer ensures easy binding of DNA to the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the ultra pure DNA is eluted by a low salt Elution Buffer or TE. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Features

  • Sample : up to 100 μl of DNA products
  • Format : spin column (centrifuge)
  • Recovery : up to 90%
  • Operation time : within 20 minutes
Applications

  • PCR
  • Fluorescent or radioactive sequencing
  • Restriction enzyme digestion
  • DNA labeling
  • Ligation
Quality Assurance

The quality of the DNA Pure Kit is tested on a lot-to-lot basis by purifying DNA of various sizes from aqueous solutions. The purified DNA is checked by electrophoresis.

Protocol

Specification
Store at

 

# FYG601-100P (100 preps)
DNA Pure Buffer 80 ml
Wash Buffer 25 ml
Elution Buffer 6 ml
DP Column 100 pcs
2 ml Collection Tube 100 pcs

*Add 100 ml of absolute ethanol to the Wash Buffer prior to initial use
 
# FYG601-300P (300 preps)
DNA Pure Buffer 240 ml
Wash Buffer 50 ml
Elution Buffer 30 ml
DP Column 300 pcs
2 ml Collection Tube 300 pcs

*Add 200 ml of absolute ethanol to the Wash Buffer prior to initial use
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