YEAtaq DNA Polymerase
Description

YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. It is able to withstand repeated heating to 95°C without significant loss of activity. The enzyme catalyzes 5’→3’ synthesis of DNA, has no detectable proofreading 3’→5’ exonuclease activity and possesses low 5’→3’ exonuclease activity. It also exhibits deoxynucleotidyl transferase activity, which normally results in the addition of extra adenines at the 3’-end of PCR products, required for the DNA ligation to TA vector.

Gentaur Biotech offers YEAtaq DNA Polymerase in two different packages: one with dNTPs mix and the other without. In addition, a lyophilized version of this enzyme will be on the market later soon this year.

The enzyme is in a recombinant form expressed in E. coli.
Features

  • Thermostable : half life is more than 40 min at 95°C.
  • 10× Reaction Buffer is supplied with two components: KCl and (NH4)2SO4, the latter allows for PCR at wide range of magnesium concentrations and decreases non-specific priming.
  • Incorporates modified nucleotides (e.g., dUTP, dITP, biotin-, digoxigenin-,fluorescently-labeled nucleotides).
  • The error rate of YEAtaq DNA Polymerase in PCR is 2×10-5 errors per nucleotide per cycle.
Applications

  • Cloning
  • Screening
  • Primer extension
  • Terminal dA tailing
  • Routine PCR amplification of DNA fragments up to 3 Kb
  • DNA labeling
  • DNA sequencing
Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.
Quality Control

  • Nuclease activity is not detected after incubation of 1 μg λ /HindIII DNA with 5 units of YEAtaq DNA polymerase in 50 μl reaction buffer for 18 hours at 37 °C.
  • The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate tests. Functional test is performed by PCR.
Patterns/Disclaimer

Some applications in which this product can be used may be covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Results

 

Specification
Store at

 

# FYT001-500U (500 units)
YEAtaq DNA Polymerase
(5 U/μl)		 100 μl
10× Reaction Buffer 2 ml
dNTPs Mix  (10 mM) 200 μl 
# FYT011-500U (500units)
YEAtaq DNA Polymerase
(5 U/μl)		 100 μl
10× Reaction Buffer 2 ml

 

Storage Buffer

50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*

100 mM KCl, 20 mM MgSO4·7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA

* The reaction buffer is supplied as a 10× concentrate and should be diluted before use.
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